五加生化胶囊对药物流产出血模型大鼠的影响及机制研究  

Protective Effect and Mechanism of Wujia Biochemical Capsule on Rats with Drug Abortion and Hemorrhage

作　　者：张智博[1] 刘斌[1] 姚远[1] 王瑶[1] 李铁男[1] 王发善[1] 张宁[1,2] ZHANG Zhi-bo;LIU Bin;YAO Yuan;WANG Yao;LI Tie-nan;WANG Fa-shan;ZHANG Ning(Heilongjiang University of traditional Chinese Medicine,Harbin,Heilongjiang,150040,China;The Key Laboratory of Ministry ofeducation and province of basic medicine and applied research of Heilongjiang University of traditional Chinese Medicine,Harbin,Heilongjiang,150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室,黑龙江哈尔滨150040

出　　处：《现代生物医学进展》2019年第4期636-641,共6页Progress in Modern Biomedicine

基　　金：黑龙江省科技攻关计划项目(GC10C208);哈尔滨市科技创新人才研究专项资金(2016RQYXJ012);黑龙江中医药大学校科研基金项目(201718;201726);黑龙江省博士后资助项目(LB-Z16200)

摘　　要：目的:观察五加生化胶囊对药物(米非司酮配伍米索前列醇)致流产出血大鼠模型的保护作用,并探讨其作用机制。方法:建立大鼠药物流产出血模型,分为正常组(K)、受孕对照组(KY)、模型组(M)和给药(G)组,观测大鼠子宫出血时间、出血量、子宫指数、子宫形态和血液流变学改变,采用放射免疫法、ELISA法、免疫组织化学法和IPP6.0图像分析系统对比观察了大鼠血清雌二醇(E2)、血浆层粘连蛋白(LM)和纤维连接蛋白(FN)含量变化,以及子宫组织中FN、LM和雌、孕激素受体(ER、PR)表达的变化。结果:与M组比较,G组药物流产出血大鼠子宫血量由0.40±0.15**mL减少到0.22±0.16#m L,出血时间由124.4±22.0**h减少到71.3±10.4##h,血清E2含量在第14天由22.47±6.37▲pg/mL到39.57±5.19##▲▲pg/mL,子宫ERα表达OD值由0.036±0.025**升高到0.208±0.072##和PR的表达OD值由0.043±0.023*升高到0.095±0.035#,血浆和子宫组织LM和FN水平降低,血液流变性得以改善。结论:五加生化胶囊具有减少药物流产出血大鼠子宫出血量和出血时间的作用,其作用机制主要为降低了血浆和子宫组织FN的含量,起到使残留的蜕膜组织顺利排出的目的,减少了残留蜕膜对子宫的刺激;提高血清中E2含量和子宫组织中ERα和PR的表达,同时改善了血液流变性,使受损子宫组织局部血液循环得到改善加速了受损子宫的恢复速度。Objective: To observe the protective effect of Wujia Shenghua Capsule on rats with hemorrhage induced by mifepristone and misoprostol and to explore its mechanism. Methods: Establishment a rat model of hemorrhage due to medical abortion, Divided into normal group(K), pregnant control group(KY), model group(M) and administration group(G), Observe the Changes of uterine bleeding time, bleeding volume, uterine index, uterine morphology and hemorheology in rats, the changes of serum estradiol(E2), plasma laminin(LM) and fibronectin(FN) in rats were observed by radioimmunoassay, immunohistochemistry and IPP6.0 image analysis system,Changes of expression of FN, LM, estrogen and progesterone receptor(ERP, PR) in uterus. Results: Compared with M group, the uterine blood volume were reduced from 0.40 ±0.15**mL to 0.22 ±0.16#m L, the bleeding time were significantly decreased from 124.4 ±22.0**h to 71.3±10.4##h, the level of serum E2 increased from 22.47±6.37 pg/m L to 39.57±5.19#pg/m L on the 14 thday, The OD value of ER α expression in uterus increased from 0.036 ±0.025* to 0.208 ±0.072##and OD value of PR expression increased from 0.043 ±0.023* to 0.095±0.03*, the levels of LM and FN in plasma and uterine tissue were decreased, and the hemorheology was improved in group G. Conclusion: Wujia Shenghua capsule can reduce the amount and time of uterine bleeding in rats with drug abortion hemorrhage, and its mechanism mainly for the FN content in plasma and uterine tissues was reduced, the residual decidual tissue was expelled smoothly, the stimulation to uterus was reduced, the E2 content in serum and the expression of ERa and PR in uterine tissues were increased, the hemorheology was improved, the local blood circulation of damaged uterine tissues was improved and accelerated, damage the recovery rate of the uterus.

关 键 词：五加生化胶囊 药物流产出血 雌二醇 雌激素受体 米非司酮 

分 类 号：R-33[医药卫生] R714.21