大瑶山甜茶正丁醇部位化学成分研究  

Chemical Studies on the n-Butanol Extract of Rubus suavissimus S. Lee

作　　者：彭玉勃 吴娇 李杨 王大利[3] 李小莹 张艳[1] PENG Yu-bo;WU Jiao;LI Yang;WANG Da-li;LI Xiao-ying;ZHANG Yan(Jiamusi college of Heilongjiang University of Chinese Medicine,Jiamusi,Heilongjiang,154007,China;College of Life Science,Dalian Nationalities University,Dalian,Liaoning,116600,China;Dalian Children's Hospital,Dalian,Liaoning,116012,China)

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007 [2]大连民族大学生命科学学院,辽宁大连116600 [3]大连市儿童医院,辽宁大连116012

出　　处：《现代生物医学进展》2019年第3期434-438,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81274035)

摘　　要：目的:研究大瑶山甜茶正丁醇部位中的二萜类化学成分,为探索其活性物质奠定基础。方法:采用95%乙醇提取药材,提取液回收至无醇味后溶于水中,依次用石油醚、乙酸乙酯和正丁醇萃取,减压干燥后获得相应萃取物,综合运用各种色谱技术对正丁醇萃取物进行分离纯化,获取二萜类化合物,通过NMR对得到的化合物进行结构鉴定。结果:从大瑶山甜茶中分离得到10个二萜类化合物,分别鉴定为7β, 17-dihydroxy-16β-ent-kauran-19-oic acid 19-O-β-D-glucopyranoside ester (1), 7β, 17-dihydroxy-entkaur-15-en-19-oic acid 19-O-β-D-glucopyranoside ester (2), 13-[(O-β-D-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid 2-O-β-Dglucopyranosyl-β-D-glucopyranosyl ester (3), 12-α-[(2-O-β-D-glucopyranosyl-β-Dglucopyranosyl)oxy]ent-kaur-16-en-19-oic acidβ-Dglucopyranosyl ester (4), glaucocalyxin G (5),β-D-glucopyranosyl 17-hydroxy-ent-kauran-19-oate-16-O-β-D-glucopyranoside (6),cussoracosides E (7), 17-O-β-D-glucopyranosyl-16α-ent-kauran-19-oic acid (8),cussovantoside A (9),cussovantoside C (10)。结论:化合物1-10首次从大瑶山甜茶中分离得到。Objective: To study the diterpenoids of n-butanol extract of Rubus suavissimus S. Lee., and to establish the foundation for its active substances. Methods: The medicinal materials were extracted by 95% Et OH. The extract was recovered to non-alcoholic taste and dissolved in water. The extract was successively extracted with petroleum ether, ethyl acetate and n-butanol. After drying under reduced pressure, the corresponding extract was obtained, and the n-butanol extract was separated and purified by various chromatographic techniques. Results: The structures of the compounds were established as 7β, 17-dihydroxy-16β-ent-kauran-19-oic acid19-O-β-D-glucopyranoside ester(1), 7 β, 17-dihydroxy-ent-kaur-15-en-19-oic acid 19-O-β-D-glucopyranoside ester(2), 13-[(O-β-Dglucopyranosyl)oxy]ent-kaur-16-en-19-oic acid 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester(3), 12-α-[(2-O-β-D-glucopyranosyl-β-Dglucopyranosyl)oxy]ent-kaur-16-en-19-oic acid β-D-glucopyranosyl ester(4), glaucocalyxin G(5), β-D-glucopyranosyl 17-hydroxyent-kauran-19-oate-16-O-β-D-glucopyranoside(6), cussoracosides E(7), 17-O-β-D-glucopyranosyl-16α-ent-kauran-19-oic acid(8),cussovantoside A(9), and cussovantoside C(10). Conclusion: Compounds 1-10 were obtained from Rubus suavissimus S. Lee firstly.

关 键 词：大瑶山甜茶 正丁醇部位 化学成分 

分 类 号：S571.1[农业科学—茶叶生产加工] R284.2[农业科学—作物学]