头孢喹肟(CEF)影响牦牛瘤胃微生态的宏基因组学分析  

Metagenomic analysis of cefquinome( CEF) affecting rumen microecology of yak

作　　者：安雅静 文勇立 赵佳琦 李子谦 齐沛森 马定美 扎升 侯定超 李强 AN Yajing;WEN Yongli;ZHA;LI Ziqian;QI Peisen;MA Dingmei;ZHA Sheng;HOU Dingchao;LI Qiang(Institute of Qinghai-Tibetan Plateau, Southwest Minzu University, Chengdu 610041,China;Aba Prefecture Jinchuan County Animal Husbandry and Veterinary Service Center, Jinchuan 624100, China;Sichuan Animal Husbandry Station, Chengdu 610041,China)

机构地区：[1]西南民族大学青藏高原研究院,成都610041 [2]阿坝州金川县畜牧兽医服务中心,四川金川624100 [3]四川省畜牧总站,成都610041

出　　处：《黑龙江畜牧兽医》2019年第5期136-141,182,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金："十二五"国家科技支撑计划项目(2014BAD13B03);西南民族大学研究生创新型科研项目(CX2017SZ024)

摘　　要：为了探讨头孢喹肟(CEF)对牦牛瘤胃微生态的影响,试验将牦牛分为处理组和对照组,处理组静脉注射CEF 1 mg/kg(按体重),对照组未做抗生素处理。采集各组瘤胃液,通过生物信息学分析软件考查CEF对瘤胃菌群多样性及碳水化合物酶类(CAZy)功能的影响。结果表明:CEF可降低拟杆菌门(Bacteroidetes)丰度,在属水平上CEF对普雷沃氏菌属(Prevotella)和拟杆菌属(Bacteroides)具有抑制作用;CEF对CAZy中糖苷水解酶(GH)、糖基转移酶(GT)、碳水化合物结合模块(CBM)等9类功能酶均产生影响,干扰碳水化合物代谢;牦牛瘤胃微生物中降解植物纤维素主要的CAZy是GH和GT家族。说明CEF可改变瘤胃细菌多样性,影响CAZy功能,使瘤胃微生态失衡。In order to investigate the effect of cefquinome (CEF) on the rumen microecology of yak, this experiment was designed by the treatment group ( CEF) and the control group ( C). The treatment group received intravenous injection of CEF 1 mg/kg ( by body weight), and the control group was not treated with antibiotics. The bioinformatics softwares were used to analyze diversity of rumen bacteria and CAZy function. The result was shown that CEF reduced the abundance of Bacteroides. At the genus level, CEF had an inhibitory effect on Prevotella and Bacteroides. The CEF affected nine functional enzymes such as Glycoside Hydrolases ( GH), Glycosyl Transferases ( GT) and Carbohydrate-Binding Modules ( CBM) and so on in carbohydrate enzymes ( CAZy), and interfered with carbohydrate metabolism. The main carbohydrate enzymes that degrade plant cellulose in rumen microbes were two families as GH and GT. Conclusion: the CEF could change the rumen bacterial diversity ,and affect the function of carbohydrate enzymes ( CAZy), and lead to the rumen micro-ecological imbalance.

关 键 词：头孢喹肟(CEF) 瘤胃 微生物多样性 宏基因组学 碳水化合物酶类 牦牛 

分 类 号：S852.6[农业科学—基础兽医学]