应用响应面法优化金针菇子实体多糖提取工艺  

Optimization of Extraction Processing of Polysaccharide from Flammulina velutipes by Response Surface Analysis

作　　者：刘晓敏 黎玉婷 沈彩奕 王溢华 冯光志 LIU Xiao-min;LI Yu-ting;SHEN Cai-yi(College of Food and Biotechnology,Wuhan Institute of Design and Sciences,Wuhan,Hubei 430205)

机构地区：[1]武汉设计工程学院食品与生物科技学院,湖北武汉430205

出　　处：《安徽农业科学》2019年第8期159-161,167,共4页Journal of Anhui Agricultural Sciences

基　　金：国家级大学生科技创新项目(201814035007);武汉设计工程学院博士科研专项(k201711)

摘　　要：采用热水浸提和乙醇沉淀法提取金针菇子实体多糖,在单因素试验的基础上,采用响应面分析法来优化多糖的工艺条件。结果表明,多糖提取的最优条件为水料比40,浸提时间2.26 h,浸提温度95℃,粗多糖得率为3.82%。The polysaccharides were prepared by hot water extraction and ethanol precipitation.The method of response surface analysis was used to optimize the technological conditions of polysaccharide on the basis of single factor test.The resultsshowed that the optimal conditions were as follows:water- material ratio 40,extraction time2.26 h,extraction temperature 95 ℃,crude polysaccharide yield 3.82%.

关 键 词：金针菇子实体多糖 BOX-BEHNKEN试验设计 热水浸提 

分 类 号：TQ914.1[化学工程]