miR-193a在肝癌中的表达水平及临床意义  

The role and clinical significance of miR-193a in hepatocellular carcinoma

作　　者：李宝华[1] 戈海泽[1] 刘树业[1] LI Baohua;GE Haize;LIU Shuye(Department of Clinical Laboratory,Tianjin Third Central Hospital,Tianjin 300170,China)

机构地区：[1]天津市第三中心医院检验科,300170

出　　处：《重庆医学》2019年第8期1334-1338,共5页Chongqing medicine

基　　金：天津市教育科学"十三五"规划课题(VE3114);天津市医院协会医院管理研究项目2016年度立项课题(2016zch04);雅培病毒性肝炎诊疗课题资助项目(2013-ADD)

摘　　要：目的探讨微RNA-193a(microRNA-193a,miR-193a)在肝癌中的表达水平,并在分子水平研究其相关作用机制。方法数据库分析和RT-PCR试验检测人肝癌组织及其癌旁组织中miR-193a的表达水平;CCK-8法检测miR-193a对Huh7细胞活性的影响;细胞集落形成和Transwell试验分别检测miR-193a对Huh7细胞集落形成、迁移和侵袭能力的影响;荧光素酶报告试验检测miR-193a与其候选靶基因KRAS的靶向关系;RT-PCR和Western blot检测miR-193a对KRAS mRNA和蛋白水平的影响。结果与癌旁组织比较,肿瘤组织中miR-193a的表达水平明显降低,这与GEO和TCGA数据库中的数据结果一致;转染miR-193a mimics的肝癌细胞Huh7的细胞活性、集落形成能力、迁移能力及侵袭能力都低于转染miR-NC组;miR-193a能够直接靶向KRAS并下调其表达。结论 miR-193a通过下调KRAS表达抑制了肝癌细胞增殖、迁移和侵袭能力。Objective To investigate the expression level of miR-193a in hepatocarcinoma and study its related mechanism at the molecular level.Methods Database analysis and RT-PCR were used to detect the expression of miR-193a in human hepatocarcinoma tissues and adjacent tissues.CCK-8 method was used to detect the effect of miR-193a on Huh7 cell activity.Cell colony formation and Transwell assay were used to detect the effect of miR-193a on colony formation,migration and invasion ability;luciferase reporter assay to detect the targeting relationship between miR-193a and its candidate target gene KRAS;RT-PCR and Western blot to detect the effect of miR-193a on KRAS mRNA and protein levels.Results Compared with paracancerous tissues,the expression level of miR-193a in tumor tissues was significantly decreased,which was consistent with the results in the GEO and TCGA databases.The cell viability,colony forming ability,migration ability and invasive ability of Huh7 transfected with miR-193a mimics was lower than that of the miR-NC group(transfected with miR-NC).miR-193a was able to directly target KRAS and down-regulate its expression.Conclusion miR-193a mimics suppressed cell proliferation,cell migration and invasion by targeting KRAS expression.

关 键 词：肝肿瘤 miR-193a HUH7细胞 KRAS 

分 类 号：R735.7[医药卫生—肿瘤]