基于2-酮基-D-葡萄糖酸高产的发酵条件优化研究  

Optimization of the Fermentation Condition for 2-Keto-D-Gluconic Acid Producing Strain

作　　者：罗秋玲[1] 刘佳[1] 张权 杨彬 陈修来[1] 刘立明[1] LUO Qiuling;LIU Jia;ZHANG Quan;YANG Bin;CHEN Xiulai;LIU Liming(State Key Lab. Food Sci. Technol.,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2019年第2期22-27,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31270079;21422602);国家973项目子课题(2013CB733602);国家863计划项目(2012AA022108;2014AA021501;2014AA021701)

摘　　要：为了实现异维生素C前体2-酮基-D-葡萄糖酸在沙雷氏菌Serratia sp. FMME043中的高效生产,在30 L发酵罐中对补料策略和发酵条件进行了优化,建立了补料分批发酵工艺。最终确定发酵策略为:溶氧控制在40%,在发酵16、22 h及28 h时分别补加64、80 g和96 g硫酸铵,并且当残糖浓度降至15～25 g/L时,分五次等量补加801 g葡萄糖,优化后,发酵44 h,2-KGA的产量和转化率分别达到268.5 g/L和1.04 g/g,分别比优化前提高了58.4%和9.9%。本研究结果为目前报道的产量和转化率在国内外属领先水平,为2-酮基-D-葡萄糖酸及异维生素C工业化生产打下了坚实的基础。In order to achieve the high production of 2-keto-D-gluconic acid(2-KGA) by Serratia sp. FMME043,feeding strategy and fermentation conditions are optimized in 30 L fermentor,and fed batch fermentation is established. The optimal fermentation conditions are as follows:the dissolved oxygen is 40%,the quality of 64,80 g,and 96 g ammonium sulfate is added at 16,22 h,and 28 h,respectively,and the quality of 801 g glucose is supplemented with five times at the residual sugar concentration between 15~25 g/L. Under these conditions,at 44 h,the production and the yield of2-KGA reached 268.5 g/L and 1.04 g/g,which are 58.4% and 9.9% higher than that not optimized,respectively.

关 键 词：沙雷氏菌属 2-酮基-D-葡萄糖酸 发酵优化 溶氧 

分 类 号：Q815[生物学—生物工程]