虹鳟肝组织新转录本分析及基因结构优化  

Analysis of novel transcripts and optimization of the gene structure in the liver of rainbow trout

作　　者：马芳[1] 刘哲[1] 康玉军[1] 权金强 MA Fang;LIU Zhe;KANG Yujun;QUAN Jinqiang(College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070

出　　处：《中国实验动物学报》2019年第2期135-142,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(31660735)~~

摘　　要：目的虹鳟热应激下肝RNA-seq数据中新转录本的分析及已注释基因结构优化。方法以虹鳟肝为材料提取总RNA,构建cDNA文库,并利用Illumina双端测序Hiseq 2500平台进行测序。运用Cufflinks软件对测序数据进行组装,将其与虹鳟参考基因组进行序列比对。结果发掘新转录本6555个,其中30个新转录本在热应激前后差异表达(P<0.05)。与GO数据库比对对新转录本进行功能注释,获得3097个新转录本的注释。与KEGG数据库比对,共有3617个新转录本注释到284条代谢通路中。对19 424个已注释基因的结构进行优化,延伸了14 719个基因的5′端和14 796个基因的3′端。结论通过对发掘的6555个新转录本分析,并对19 424个已注释基因结构优化,为虹鳟基因组注释信息的完善提供了有力的借鉴,并为进一步了解虹鳟热应激的机制提供更有力的理论基础。Objective To discover novel transcripts from RNA-seq data of rainbow trout under heat stress and optimize the annotated gene structure. Methods Total RNA was extracted from the liver of rainbow trout and used to construct a cDNA library that was sequenced by the Illumina two-terminal sequencing Hiseq 2500 platform. Sequencing data were assembled by Cufflinks software and aligned with the rainbow trout genome. Results Among 6555 new transcripts, 30 were differentially expressed under heat stress ( P < 0.05). A total of 3097 novel transcripts were annotated and blasted by the GO database. There were 3617 new transcripts annotated in 284 metabolic pathways by KEGG database. The structure of 19 424 annotated genes was optimized, and the 5′-ends of 14 719 genes and 3'-ends of 14 796 genes were extended. Conclusions Discovery and analysis of 6555 novel transcripts and 19 424 gene structure optimization provide a powerful reference optimizing the genome annotation information of rainbow trout, and a more powerful theoretical basis for further understanding the mechanism of heat stress in rainbow trout.

关 键 词：虹鳟 转录组测序 新转录本 基因结构优化 

分 类 号：Q95-33[生物学—动物学]