苏州地区汉族人群19个常染色体STR基因座的遗传多态性研究  

Genetic polymorphisms of 19 autosomal STR loci in Suzhou Han population

作　　者：吕文锐 徐家斌 蓝焘 周玮[1,4] 陶瑞 冯雪松 高玉振[1,4] LYU Wenrui;XU Jiabin;LAN Tao;ZHOU Wei;TAO Ruiyang;FENG Xuesong;GAO Yuzhen(Institute of Forensic Science,Soochow University,Suzhou 215123;Public Security Bureau of Xinghua,Jiangsu Province;Public Security Bureau of Hai'an,Nantong,Jiangsu Province;Department of Forensic Science,Medical School of Soochow University;Institute of Forensic Medicine,West China School of Basic Medical Sciences & Forensic Medicine,Sichuan University;Public Security Bureau of Qingpu,Shanghai)

机构地区：[1]苏州大学司法鉴定中心,苏州215123 [2]江苏省兴化市公安局刑警大队 [3]江苏省南通市海安县公安局 [4]苏州大学法医学系 [5]四川大学基础与法医学院 [6]上海市公安局青浦分局

出　　处：《南通大学学报（医学版）》2019年第1期1-6,共6页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金面上资助项目(81572767)

摘　　要：目的:调查19个常染色体短串联重复序列(short tandem repeat, STR)基因座在中国苏州地区汉族人群中的遗传多态性,考察其在法医遗传学相关鉴定中的应用价值,并为涉及该地区的群体遗传学研究提供基础数据支持。方法:采用AGCU Expressmarker 20荧光检测试剂盒对1 050名中国苏州地区汉族无关个体进行STR扩增及检测,根据各基因座分型结果计算了法医遗传学相关参数,并分析了苏州汉族与其他地区汉族在遗传距离上的相近程度。结果:经Bonferroni校正后,19个常染色体STR基因座均符合Hardy-Weinberg平衡(P>0.05/19=0.002 6),累积个人识别概率达到0.999 999 999 999 999,二联体累积非父排除概率为0.999 992 521 059 119,三联体累积非父排除概率为0.999 999 997 756 847。多维尺度分析显示苏州汉族同其他地区汉族人群遗传距离基本呈现与地理位置分布相符合的特征。结论 :本研究纳入的19个常染色体STR基因座在中国苏州地区汉族人群中具有较高的遗传多态性,可应用于法医遗传学相关鉴定和群体遗传学研究。Objective : To investigate the genetic polymorphisms of 19 autosomal STR loci in Suzhou Han population and to explore the application values in forensic practice, in order to provide fundamental data for studies concerning population genetics of this region. Methods : A total of 1 050 unrelated Han individual samples in Suzhou were collected and amplified using the AGCU Expressmarker 20 system kit. The forensic parameters were calculated based on the raw genotyping data obtained and the population comparation was conducted based on the genetic distances between different population. Results : No significant deviation from Hardy-Weinberg e quilibrium w as o bserved f or t he g enotype f requency d istribution o f a ll s tudied l oci a fter Bonferroni's correction(P>0.05/19=0.002 6). The cumulative power of discrimination, probability of exclusion for diad and triplet were 0.999 999 999 999 999, 0.999 992 521 059 119 and 0.999 999 997 756 847, respectively. Besides, the multidimensional scaling analysis revealed that the genetic distances between Suzhou Han and other Han population were basically in accordance with their geographic distributions. Conclusion : The 19 autosomal STR loci enrolled in our study exhibited relatively high genetic polymorphisms in Suzhou population, which could imply a strong validity for forensic identification, paternity testing and population genetic research.

关 键 词：法医遗传学 遗传多态性 短串联重复序列 苏州 汉族 

分 类 号：DF795.2[医药卫生—法医学]