施万细胞通过NT-3/TrkC信号通路促进SACC嗜神经侵袭的研究  

Schwann cells promote the perineural invasion of salivary adenoid cystic carcinoma via NT-3/TrkC signaling pathway

作　　者：郭佳 李欢 王珺 雷德林 杨新杰 GUO Jia;LI Huan;WANG Jun;LEI Delin;YANG Xinjie(State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department Oral and Maxillofacial Surgery,School of Stomatology,The Fourth Military Medical University,710032,China)

机构地区：[1]军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学口腔医院口腔颌面外科,西安710032

出　　处：《实用口腔医学杂志》2019年第2期176-180,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81302352;81372901)

摘　　要：目的:研究共培养状态下施万细胞(SCs)对唾液腺腺样囊性癌细胞(SACCs)侵袭、迁移能力的影响及可能机制。方法:用ELISA等检测NT-3、TrkC在共培养状态下的表达情况;通过划痕、侵袭、迁移实验和3D共培养实验,评估各组细胞的侵袭与迁移能力。结果:共培养条件下,NT-3,TrkC表达较单独培养明显升高;SACC-83细胞和SCs的侵袭、迁移能力均显著增强;SCs可早期定向趋化迁移至肿瘤。结论:NT-3/TrkC信号通路可能通过介导SCs向肿瘤细胞的迁移来促进SACC嗜神经侵袭的发生。Objective:To investigate potential mechanism of the perineural invasion and motor ability of the Schwann cells(SCs)on the salivary adenoid cystic carcinoma cells(SACCs).Methods:SCs and SACCs were respectively cultured and co-cultured.The expression of NT-3 and TrkC was measured by ELISA.Wound healing test,invasion and motor test and three-dimensional(3 D)co-culture assays were performed to evaluate the motor ability under the interaction of SACC-83 cells and SCs.Results:The expression of NT-3 and TrkC were markedly elevated and the migration,and invasion ability increased significantly when cells were co-cultured.Moreover,SCs firstly migrated to SACC-83 cells before the tumor attacked the nerve.Conclusion:NT-3/TrkC axis may possibly mediate the migration of SCs towards SACC cells to promote the occurrence of PNI.

关 键 词：唾液腺腺样囊性癌 施万细胞 NT-3 TRKC 嗜神经侵袭 

分 类 号：R739.8[医药卫生—肿瘤]