膀胱癌O^6-甲基鸟嘌呤-DNA转移酶基因启动子甲基化及其临床意义  

Methylation of O^6-methylguanine-DNA transferase gene promoter in bladder cancer and its clinical significance

作　　者：刘晓丽[1] 单晓辉 王桃霞[1] 冯淑宁 王晓英[1] 段小婷 李桂英[1] LIU Xiaoli;SHAN Xiaohui;WANG Taoxia;FENG Shuning;WANG Xiaoying;DUAN Xiaoting;LI Guiying(Department of Nephrology, Affliated Hospital of Hebei University of Engineering, Hebei Province, Handan 056002, China;Department of Neurology, the First Hospital of Yongnian District Handan, Hebei Province, Handan 057150, China)

机构地区：[1]河北工程大学附属医院肾内科,河北邯郸056002 [2]河北省邯郸市永年区第一医院神经内科,河北邯郸057150

出　　处：《中国医药导报》2019年第8期22-25,共4页China Medical Herald

基　　金：河北省医学科学研究重点课题计划项目(20170854)

摘　　要：目的研究膀胱癌O^6-甲基鸟嘌呤-DNA转移酶(MGMT)基因启动子甲基化情况,探讨其与临床病理特征间的关系及临床意义。方法选取2010年1月～2012年1月于河北工程大学附属医院就诊并行手术切除治疗的膀胱癌患者93例,选取同期就诊且经膀胱镜检查证实无膀胱肿瘤的47例患者作为对照。应用甲基化特异聚合酶链式反应(PCR)检测膀胱癌组织和正常膀胱黏膜组织中MGMT基因甲基化状况。统计分析MGMT甲基化与临床病理特点关系,Kaplan-Meier分析MGMT甲基化与患者预后间的关系。结果膀胱癌组织中MGMT相对甲基化程度明显高于正常膀胱组织,差异有统计学意义(P <0.01)。MGMT甲基化与分期、分级相关(均P <0.01),与年龄、性别、肿瘤大小、数目、形态及复发频率无关(均P> 0.05)。Kaplan-Meier生存分析发现甲基化组患者5年总体生存率明显低于非甲基化组(均P> 0.05)。结论膀胱癌组织中MGMT基因甲基化水平明显增高,MGMT甲基化与肿瘤分期、分级及不良预后有关,可作为判断膀胱癌病情判断及预后的分子生物学标志,值得临床关注。Objective To investigate the methylation of O6-methyl guanine-DNA methyl transferase (MGMT) gene promoter in bladder cancer was studied. Methods Ninety-three patients cases of bladder cancer patients admitted to the Affiliated Hospital of Hebei University of technology from January 2010 to January 2012 and treated with surgical resection were selected. Forty-seven cases of bladder cancer patients Admitted to the Hospital during the same period and confirmed to have no bladder tumor by cystoscopy were selected as the control group. Methylation of MGMT gene in bladder cancer and normal bladder mucosa were detected by methylation specific polymerase chain reaction (PCR). The correlation between MGMT methylation and clinical-pathological characteristics was statistically analyzed, and Kaplan-Meier was used to analyze the correlation between MGMT methylation and patient prognosis. Results The relative methylation of MGMT in bladder cancer tissues was significantly higher than that in normal bladder tissues, and the difference was statistically significant (P < 0.01). MGMT methylation was correlated with staging and grading (all P < 0.01), but not with age, gender, tumor size, number, morphology and recurrence frequency (all P > 0.05), Kaplan-Meier survival analysis found that the 5-year overall survival rate of the methylated group was significantly lower than that of the non-methylated group (all P > 0.05). Conclusion The methylation level of MGMT gene was significantly increased in bladder cancer tissue, and MGMT methylation was related to tumor stage, grade and poor prognosis, which could be used as a molecular marker for the diagnosis and prognosis of bladder cancer and worthy of clinical attention.

关 键 词：膀胱癌 O6-甲基鸟嘌呤-DNA转移酶 甲基化 临床意义 

分 类 号：R737.14[医药卫生—肿瘤]