羌活中香豆素类化学成分及其抗氧化活性研究  

Chemical constituents of coumarins compounds from Notopterygium incisum and their anti-oxidant activity

作　　者：刘文武 蒋晓文[2] 张帅 祖宇欣 赵庆春 LIU Wen-wu;JIANG Xiao-wen;ZHANG Shuai;ZU Yu-xin;ZHAO Qing-chun(Department of Pharmacy, General Hospital of Northern Theater Command, Shenyang 110840, China;Shenyang Pharmaceutical University, Shenyang 110016, China)

机构地区：[1]北部战区总医院药剂科,辽宁沈阳110840 [2]沈阳药科大学,辽宁沈阳110016

出　　处：《中草药》2019年第6期1310-1315,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81673328)

摘　　要：目的研究羌活Notopterygium incisum中的香豆素类化学成分及其抗氧化活性。方法采用重结晶、硅胶柱色谱、SephadexLH-20柱色谱以及制备HPLC等多种分离手段进行分离纯化,通过现代波谱学方法鉴定结构,并采用DPPH法和ABTS法对分离得到的化合物进行抗氧化活性测试。结果从羌活70%乙醇提取物中的二氯甲烷萃取层分离得到10个化合物,分别鉴定为佛手酚(1)、中甸前胡素(2)、镰叶芹二醇(3)、苯乙基阿魏酸酯(4)、丝立尼亭(5)、拱当归素(6)、羌活酚(7)、紫花前胡苷元(8)、(+)-cis-khellactone(9)、水合氧化前胡素(10),并经2种不同的方法测试了这类化合物的抗氧化活性。结论化合物2、6为首次从该植物中分离得到;经DPPH法和ABTS法测试发现化合物1和7具有较强的抗氧化能力;构效关系分析发现5位上具有-OH,多烯烃结构的呋喃环香豆素具有比其他香豆素类化合物更强的抗氧化活性。Objective To investigate the coumarin compounds from Notopterygium incisum and their anti-oxidant activities.Methods The coumarin compounds and their analogues were separated and purified by recrystallization, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. The structure was identified by modern spectroscopy. The isolated compound was tested for anti-oxidant activity by DPPH and ABTS assay. Results Ten compounds were isolated from the dichloromethane extraction layer of 70% ethanol extract, which were identified as bergaptol(1), d-laserpitin(2),falcarindiol(3), phenethyl ferulate(4), selinidin(5), archangelicin(6), notoptol(7), nodakenetin(8),(+)-cis-khellactone(9), and oxypeucedanin hydrate(10). The anti-oxidant activity of these compounds was tested by DPPH and ABTS methods. Conclusion Compounds 2 and 6 are isolated from this plant for the first time. Compounds 1 and 7 have shown the strongest anti-oxidant activity.The structure-activity relationship(SAR) studies revealed the-OH and unsaturated double bond on C-5′ in the aromatic ring significantly improved the anti-oxidant activity than other coumarin compounds.

关 键 词：羌活 香豆素类 佛手酚 中甸前胡素 拱当归素 羌活酚 抗氧化活性 构效关系 

分 类 号：R284.1[医药卫生—中药学]