RNA-seq analysis for soybean cyst nematode resistance of Glycine soja(wild soybean)  

作　　者：Cuiping Yuan Ling Zhang Hongkun Zhao Yumin Wang Xiaodong Liu Yingshan Dong Glen L. Hartman 

机构地区：[1]Soybean Research Institute, Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean [2]Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences [3]Crop Germplasm Institute, Jilin Academy of Agricultural Sciences [4]United States Department of Agriculture-Agricultural Research Service

出　　处：《Oil Crop Science》2019年第1期32-46,共15页中国油料作物学报(英文版）

基　　金：supported by Science and Technology Development Program of Jilin Province (20170414009GH);Agricultural Science and Technology Innovation Project of Jilin Province (CXGC2017JQ018, CXGC2017ZY024);the United States Department of Agriculture-Agricultural Research Service

摘　　要：To explore genetic resource of wild soybean(Glycine soia. L), RNA-seq was used to investigate cyst nematode resistance of G. soja. Root transcriptome expressions were profiled at 9, 15 and 20 d post inoculation(DPI) in resistant and susceptible G. soja to SCN(soybean cyst nematode). A total of 1,594 differentially expressed genes(DEGs) were identified in roots infected by SCN compared with non-infected roots. In the resistant accession, 619, 65, and 8 DEGs were detected at 9, 15, and 20 DPI, respectively, while 327, 460 and 115 DEGs were detected at the same sampling point of susceptible accessions. DEGs were enriched in peroxidase gene sets which were involved in response to oxidative stress and oxidation reduction. Two gene families, ZIM transcription factor and WRKY transcription factor were enriched. WRKY transcription factor was only enriched in resistant accession. Moreover, gene expressions of 9 DEGs were validated by qRT-PCR. XLOC_023202, an unknown protein was up regulated more than 5 fold at 9 and 15 DPI in the resistant accession. These results provided an atlas of gene expressions of G. soja in response to SCN infection, and identified candidate DEGs for future research.To explore genetic resource of wild soybean(Glycine soia. L), RNA-seq was used to investigate cyst nematode resistance of G. soja. Root transcriptome expressions were profiled at 9, 15 and 20 d post inoculation(DPI) in resistant and susceptible G. soja to SCN(soybean cyst nematode). A total of 1,594 differentially expressed genes(DEGs) were identified in roots infected by SCN compared with non-infected roots. In the resistant accession, 619, 65, and 8 DEGs were detected at 9, 15, and 20 DPI, respectively, while 327, 460 and 115 DEGs were detected at the same sampling point of susceptible accessions. DEGs were enriched in peroxidase gene sets which were involved in response to oxidative stress and oxidation reduction. Two gene families, ZIM transcription factor and WRKY transcription factor were enriched. WRKY transcription factor was only enriched in resistant accession. Moreover, gene expressions of 9 DEGs were validated by qRT-PCR. XLOC_023202, an unknown protein was up regulated more than 5 fold at 9 and 15 DPI in the resistant accession. These results provided an atlas of gene expressions of G. soja in response to SCN infection, and identified candidate DEGs for future research.

关 键 词：G.soja SCN DIFFERENTIAL expression RNA-SEQ CYST NEMATODE 

分 类 号：S[农业科学]