头穴丛刺联合多奈哌齐对阿尔茨海默病大鼠海马及皮层区ChAT及AchE活性的影响  

Effect of cluster needling of scalp point therapy combinded with donepezil on the activities of choline acetyl transferase and choline cholinesterase in hippocampus and cerebral cortex of rats with Alzheimer's disease

作　　者：李虹霖[1] 高伟 孙琦月 佟晓薇 栾凯迪 徐飞 朱红奇 齐慧敏 朱斌 夏昆鹏[1] LI Hong-lin;GAO Wei;SUN Qi-yue;TONG Xiao-wei;LUAN Kai-di;XU Fei;ZHU Hong-qi;QI Hui-min;ZHI Bin;XIA Kun-peng(Department of Rehabilitation,the Second Hospital Affiliated to Heilongjiang University of Traditional Chinese Medicine,Harbin 150000,China)

机构地区：[1]黑龙江中医药大学附属第二医院康复科三病房,黑龙江省哈尔滨市150000

出　　处：《实用老年医学》2019年第4期324-327,共4页Practical Geriatrics

基　　金：黑龙江省自然科学基金青年基金(QC2017116);哈尔滨市科技局科技创新人才课题(2016RAQXJ164);黑龙江中医药大学附属第二医院科研项目基金(YZ-201701);黑龙江中医药大学研究生创新科研项目(yjscx2017045)

摘　　要：目的探讨头穴丛刺联合多奈哌齐对阿尔茨海默病(Alzheimer's disease,AD)大鼠海马及皮层区乙酰胆碱转移酶(ChAT)、乙酰胆碱酯酶(Ach E)活性的影响。方法海马区注射Aβ1-42诱导AD模型,60只Wistar大鼠随机分为假手术组、模型组、多奈哌齐组(对照组)、头穴丛刺联合多奈哌齐组(治疗组),每组15只,Morris水迷宫检测大鼠学习记忆能力; HE染色观察海马及皮层区大体结构的变化;比色法观察海马及皮层区ChAT、AchE活性变化。结果对照组海马及皮层区ChAT水平显著高于模型组、AchE水平低于模型组(均P<0. 05);治疗组海马及皮层区ChAT水平均显著高于对照组(P<0. 01); AchE水平低于对照组(P<0. 05)。结论头穴丛刺联合多奈哌齐对AD大鼠海马及皮层区ChAT的提高及AchE活性的下调是治疗AD的机制之一,头穴丛刺联合多奈哌齐疗效更佳。Objective To investigate the effect of cluster needling of scalp point therapy on the activities of choline acetyltransferase(ChAT)and acety cholinesterase(AchE)in hippocampus and cerebral cortex of rats with Alzheimer's disease(AD).Methods Models of AD was established by injection of Aβ1-42 oligomer into bilateral hippocampues.Sixty Wistar rats were randomly divided into four groups including model group,sham operation group,control group and observation group,with 15 rats in each group.Model group and sham operation group were given the same way and fixed grip,but without any treatment.The control group received donepezil and the observation group was treated by cluster needling of scalp point as well as donepezil.After the treatment for four weeks,Morris water maze was used to monitor the spatial discrimination.Colorimetric method was used to detect the activity of ChAT and AchE,and the general structure of hippocampus was observed by HE staining.Results The level of ChAT in hippocampus and cortex of the control group were more significantly increased,and the level of AchE was decreased more significantly than those of the model group(P<0.01);The level of ChAT was higher and the level of AchE was lower in treatment group than those in the control group(P<0.05).Conclusions The treatment of cluster needling of scalp point therapy combined with Donepezil can increase the activity of ChAT and decrease the activity of AchE in hippocampus and cerebral cortex of rats with AD,which is better than the effect of donepezil lonely.

关 键 词：头穴丛刺 多奈哌齐 阿尔茨海默病 海马 皮层 乙酰胆碱酯酶 乙酰胆碱转移酶 

分 类 号：R749.1[医药卫生—神经病学与精神病学]