黄体酮对成年斑马鱼下丘脑-垂体-性腺轴相关基因转录表达的干扰效应  

The effects of progesterone on the mRNA expression of genes involved in hypothalamic-pituitary-gonadal axis in adult zebrafish

作　　者：梁燕秋 董忠典 黄国勇 田斐 李进[1] 应光国[2,3] LIANG Yanqiu;DONG Zhongdian;HUANG Guoyong;TIAN Fei;LI Jin;YING Guangguo(Guangdong Ocean University,Zhanjiang 524088,China;Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou 510640,China;South China Normal University,Guangzhou 510631,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China)

机构地区：[1]广东海洋大学,湛江524088 [2]中国科学院广州地球化学研究所,广州510640 [3]华南师范大学,广州510631 [4]中国水产科学研究院南海水产研究所,广州510300

出　　处：《生态科学》2019年第2期1-8,共8页Ecological Science

基　　金：广东省自然科学基金项目(2017A030310662;2017A030313220;2018A030307020);国家自然科学基金项目(4180060608);广东海洋大学科研启动费资助项目(R17075)

摘　　要：黄体酮(P4)是一种类固醇激素。为了探究P4的内分泌干扰效应,选择成年斑马鱼(Danio rerio)作为受试生物,研究了P4对斑马鱼下丘脑-垂体-性腺轴(HPG轴)相关基因转录表达影响。成年斑马鱼在不同浓度P4(2、11和16 ng·L^(-1))下处理21 d。结果显示:暴露于高浓度组的P4能够抑制雌鱼大脑中促性腺激素释放激素2(gnrh2)、促性腺激素释放激素3(gnrh3),卵泡刺激素(fshb)、雌激素受体1(esr1)基因的转录表达;然而诱导了雄鱼大脑中fshb、黄体生成素(lhb)、雄激素受体(ar)基因的转录表达,这些转录变化暗示了P4对成年斑马鱼有潜在的弱雄激素效应。此外, P4暴露对雌鱼卵巢和雄鱼精巢类固醇合成途径中固醇激素合成急性调节蛋白(star)、细胞色素p450介导侧链裂解酶(cyp11a1)、17α羟化酶(cyp17)、卵巢细胞色素P450芳香化酶(cyp19a1a)、11β羟化酶(cyp11b)、羟基类固醇3β脱氢酶(hsd3b)、羟基类固醇20β脱氢酶(hsd20b)、羟基类固醇17β脱氢酶3(hsd17b3)、羟基类固醇11β脱氢酶2(hsd11b2)以及受体信号途径中孕激素受体(pgr)、esr1、ar基因的转录表达没有显著影响。可见,在P4暴露下,斑马鱼大脑比性腺更加敏感。总而言之, P4能够改变斑马鱼大脑中HPG轴相关基因的转录表达水平,进而对斑马鱼的内分泌系统具有潜在的危险。Progesterone(P4)is a steroid hormone.In order to reveal the endocrine disrupting effects of P4,the adult zebrafish(Danio rerio)was exposed to 2,11 and 16 ng·L^-1 P4 for 21 days,and the transcriptional expression profiles along the hypothalamic-pituitary-gonadal(HPG)axis were determined.The results showed that the highest P4 exposure led to a significant down-regulation of gonadotropin-releasing hormone 2(gnrh2),gonadotropin-releasing hormone 3(gnrh3),follicle stimulating hormone beta polypeptide(fshb)and estrogen receptor 1(esr1)genes in the brains of females,and up-regulation of fshb,luteinizing hormone beta polypeptide(lhb)and androgen receptor(ar)genes in the brains of males.These transcriptional alterations implied that P4 could exhibit the potent weak androgenic activities in adult zebrafish.In addition,P4 exposure had no significant effects on the transcriptional profiles of target genes related to the steroidogenic pathways[steroidogenic acute regulatory protein(star),cytochrome P450-mediated side-chain cleavage enzyme(cyp11a1),17-alpha-hydroxylase(cyp17),ovarian cytochrome P450 aromatase(cyp19a1a),1-beta-hydroxylase(cyp11b),hydroxysteroid 3-beta dehydrogenase(hsd3b),hydroxysteroid 20-beta dehydrogenase(hsd20b),hydroxysteroid 17-beta dehydrogenase type 3(hsd17b3)and hydroxysteroid 11-beta dehydrogenase 2(hsd11b2)]and hormone receptor genes[progesterone receptor(pgr),esr1 and ar]in the ovaries of females and testes of males.Therefore,the brain was more sensitive to P4 exposure than the gonad in zebrafish.Taken together,the overall results demonstrated that P4 could significantly affect transcriptional expression levels of genes related to HPG axis in the brains,which may pose potential adverse effects to the endocrine system of zebrafish.

关 键 词：孕激素 黄体酮 内分泌干扰 基因转录表达 

分 类 号：X174[环境科学与工程—环境科学]