单程直驱超高压纳升泵的研制与评价  

Development and evaluation of a single stroke direct-driven ultrahigh pressure nano pump

作　　者：杨三东[1,2] 董智勇 韩雪 马周 唐涛 王风云 李彤[2,3] YANG Sandong;DONG Zhiyong;HAN Xue;MA Zhou;TANG Tao;WANG Fengyun;LI Tong(Institute of Industrial Chemistry,Nanjing University of Science and Technology,Nanjing 210094,China;Dalian Elite Analytical Instruments Co.,Ltd.,Dalian 116023,China;Dalian Engineering Research Center of Chromatography,Dalian 116023,China)

机构地区：[1]南京理工大学工业化学研究所,江苏南京210094 [2]大连依利特分析仪器有限公司,辽宁大连116023 [3]大连市色谱工程技术研究中心,辽宁大连116023

出　　处：《色谱》2019年第5期558-564,共7页Chinese Journal of Chromatography

基　　金：国家重大科学仪器设备开发专项(2012YQ12004403);大连市重点领域创新团队计划项目(2016RT03)~~

摘　　要：随着生命科学的发展,纳升液相色谱系统在生化分析领域有着越来越多的应用。纳升流速输液泵作为系统的关键部件之一,其性能直接影响分析结果的准确性与重复性。该文基于高精度直驱电机和十通切换阀研制了一种单程直驱超高压纳升泵,并对其进行了评价。测试结果表明,该纳升输液泵在500 nL/min下的流速准确性优于1%,稳定性优于0.7%,最大输液压强超过100 MPa,梯度输液偏差低于1%。进一步利用该纳升输液泵构建纳升液相色谱-质谱联用系统,对1μg牛血清白蛋白(BSA)酶解液进样分析,序列覆盖率可达45%,分析1.25μg Hela细胞蛋白质酶解液鉴定到2 809个蛋白质。说明构建的单程直驱超高压纳升泵能够用于生化分析,尤其是蛋白质组学分析研究中。With the development of life science,nano liquid chromatography systems are being involved in various applications in the field of biochemical analysis.Being one of the key components in the system,the nano flow rate pump directly affected the accuracy and repeatability of the analysis.Based on two high precision direct-driven motors and a ten-port switch valve,a single stroke direct-driven ultrahigh pressure nano pump was developed.The results show that the accuracy of the flow rate and stability were better than 1% and 0.7%,respectively,at 500 nL/min.The maximum operating pressure was more than 100 MPa,and the gradient deviation was lower than 1%.The nano pump was used to construct a nano liquid chromatography-mass spectrometry system.A bovine serum albumin (BSA) digest (1 μ g) was analyzed by the system,and a sequence coverage of 45% was achieved.Furthermore,2 809 proteins were identified from a 1.25 μ g Hela cell digest.All these results demonstrated that the single stroke direct-driven ultrahigh pressure nano pump could be a useful tool in the biochemical analyses,especially for proteome research.

关 键 词：纳升液相色谱系统 直驱 超高压 纳升泵 蛋白质组学 

分 类 号：O658[理学—分析化学]