纤维蛋白原Bβ链HinfⅠ基因多态性与胃癌的相关性  

Relevance between Fibrinogen β Chain- HinfⅠGene Polymorphisms and Gastric Cancer

作　　者：严芝强[1] 杨芳[2] YAN Zhiqiang;YANG Fang(Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou, China;Guizhou Medical University, Guiyang 550004, Guizhou, China)

机构地区：[1]贵州医科大学附院,贵州贵阳550004 [2]贵州医科大学,贵州贵阳550004

出　　处：《贵州医科大学学报》2019年第4期423-426,452,共5页Journal of Guizhou Medical University

基　　金：贵州省科技厅联合基金[黔科合LJ(2016)7235号]

摘　　要：目的:探讨纤维蛋白原B(FgB)β链-HinfⅠA/C基因多态性与胃癌的相关性。方法:151例经病理确诊的胃癌患者为胃癌组,151例同期健康体检者为对照组,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测2组受检查全血DNA FgBβ-HinfⅠ位点基因型,比较两组受粒者FgBβHinfⅠC基因多态性,分析FgBβHinfⅠC基因型分布与胃癌的临床分期及转移的关系,采用logistic回归分析FgBβHinfⅠC基因型与胃癌的相对危险度。结果:FgBβHinfⅠA/C位点AA、AC、CC基因型分布及A/C等位基因频率与正常对照组比较差异具有统计学意义(P<0.05),FgBβHinfⅠA/C位3种基因型分布和等位基因频率与胃癌的临床分期及转移比较,差异无统计学意义(P>0.05);FgBβHinfⅠA/C位点AC基因型与胃癌的相对危险度分析,结果显示,OR=1.888、95%置信区间CI为1.105～3.224。结论:FgBβ-HinfⅠA/C的AC基因型可能增加胃癌的发病风险,C等位基因可能是胃癌发病的致病基因。Objective: To investigate relevance between Fibrinogen B beta chain-HinfⅠgene polymorphism and gastric cancer. Methods: 151 cases of pathological diagnosed gastric cancer patients as gastric cancer group, and 151 cases of healthy physical check takers as control group;the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technology was adapted to detect Fibrinogen beta chain-HinfⅠ site genotype. Results: Gastric cancer group fibrinogen beta beta-Hinf I A/C locus AA, AC, CC genotype distribution and A/C allele frequency showed significant difference with normal control group(P<0.05);Fibrinogen beta-HinfIA/C locus of three kinds of genotype distribution and allele frequency was compared with clinical staging and transfer of gastric cancer, comparison was statistically significant(P>0.05);analysis between Fibrinogen beta-HinfI A/C locus AC genotype and relative risk degree of gastric cancer showed odds ratio(OR) as 1.888 and 95% confidence interval(CI) between 1.105 and 3.224. Conclusions: FgB beta Hinf Ⅰ AC of the A/C genotype may increase the risk of gastric cancer, C allele may be disease genes of gastric cancer.

关 键 词：胃肿瘤 纤维蛋白原 基因 多态性 限制性片段长度 

分 类 号：R735.2[医药卫生—肿瘤] R34[医药卫生—临床医学]