MMP-2和MMP-9在声带息肉中表达的比较及其相关性研究  

Comparison and correlation analysis of matrix metalloproteinase-2(MMP-2) and matrixmetalloproteinase-9(MMP-9) expression in vocal fold polyps

作　　者：宁金梅 吴平[2] 钮燕[2] 赵树波 孙勇 Ning Jinmei;Wu Ping;Niu Yan(Dept.of Otolaryngology, Qujing 1st People's Hospital, Qujing Yunnan 655000,China;Department of Otorhinolaryngology, the Second Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650101,China)

机构地区：[1]曲靖市第一人民医院耳鼻咽喉科,云南曲靖655000 [2]昆明医科大学第二附属医院耳鼻喉科,云南昆明650101

出　　处：《云南医药》2019年第2期101-105,共5页Medicine and Pharmacy of Yunnan

摘　　要：目的检测基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)在声带息肉和正常声带组织中的表达并行定量分析,并对声带息肉中的MMP-2和MMP-9进行定量比较及相关性分析,探讨声带息肉的发病机理,了解MMP-2和MMP-9在声带息肉发病机理中的作用和相互关系,为临床防治声带息肉提供理论依据.方法选取经病理诊断确诊,且排除其他声带病变的声带息肉患者标本35例为实验组.取正常声带组织标本18例为对照组.所有标本经HE染色后,进行MaxVisionTM免疫组织化学染色,用PBS(磷酸盐缓冲液)代替一抗作阴性对照,并行计算机图像定量分析.结果声带息肉组MMP-2和MMP-9阳性单位均值均高于正常声带组,有统计学差异(P<0.05);声带息肉组中MMP-9与MMP-2阳性单位均值比较,有统计学差异(P<0.05),MMP-9阳性单位均值(42.9661±9.80116)>MMP-2(30.5666±7.18438).而用声带息肉中MMP-9和MMP-2阳性单位均值做散点图,两变量之间不存在线性趋势,说明没有相关性.结论 MMP-2和MMP-9与声带息肉的发生有关,与MMP-2相比,MMP-9可能起到了更重要的作用.对于声带息肉病人,同时抑制MMP-2和MMP-9,可望成为一种更好的治疗方案.Objective The objective of this study was investigation and quantitative analysis of the expression of MMP-2 and MMP-9 in vocal fold polyps and normal tissue of vocal folds ,and quantitative comparison and correlation analysis of MMP-2 and MMP-9 expression in vocal cord polyps to probe the pathogeny of vocal fold polyp, understand the roles and interrelation of MMP-2 and MMP-9 in the pathogenesis of vocal cord polyp, and provide a theoretical basis for clinical prevention and treatment of vocal cord polyp. Methods 35 patients without other vocal cord lesions who were undergone polypectomy were enrolled in this study for the experimental group. The specimens of vocal cord polyps were diagnosed by pathologists. Specimens of normal vocal fold were obtained from 18 autopsy cases for the control group. All samples were stained with hematoxylin - eosin,and immunohistochemical staining of MMP-9 was performed with monoclonal antibodies by MaxVisionTM method, and then the average values of Positive units that MMP-9 had expressed in vocal fold polyps and normal tissue of vocal folds were analyzed by means of quantitative analysis of computer images. Negative controls were run without the primary antibody, which was replaced by PBS. Results The average values of MMP-2 and MMP-9 positive units in the vocal cord polyp group were higher than the normal vocal group, there were statistically significant differences (P<0.05). It was statistically different that the average value of MMP-9 positive units compared with MMP-2 in the vocal cord polyp group(P<0.05),and the mean of MMP-9 positive units (42.9661±9.80116) was higher than the mean of MMP-2 positive units (30.5666±7.18438). The average values of MMP-9 and MMP-2 positive units in the vocal cord polyp group were analyzed with scatter,the two variables were not linear trend shown that there was no correlation between them.Conclusion MMP-2 and MMP-9 concern with the occurrence of vocal cord polyp, compared with MMP-2, MMP-9 may play a more important role. For the patients wit

关 键 词：基质金属蛋白酶-2 基质金属蛋白酶-9 声带息肉 免疫组织化学 图像定量分析 细胞外基质 基底膜 

分 类 号：R737.11[医药卫生—肿瘤]