原代神经小胶质细胞培养方法的初探  

A preliminary study of primary microglia cells separation and purification

作　　者：廖婷[1] 袁雪 荣曦[1] 刘红[1] Liao Ting;Yuan Xue;Rong Xi;Liu Hong(Department of Geriatric Endocrinology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院老年内分泌科,南宁530021

出　　处：《广西医科大学学报》2019年第4期650-653,共4页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81760154);国家自然科学基金青年科学基金资助项目(No.31600677);广西自然科学基金资助项目(No.2017JJA10168);大学生创新创业课题资助项目(No.201710598006;No.201810598030)

摘　　要：目的:建立一种简单有效的小胶质细胞原代培养和纯化方法。方法:取出生24h的SD大鼠的脑皮层组织,胰酶消化成单个细胞、培养,待细胞长满培养瓶后,用力拍打培养瓶3min,离心并收集沉淀,加入新培养基再培养,采用免疫荧光法鉴定其纯度。结果:共聚焦显微镜鉴定小胶质细胞纯度达到96.85%。结论:拍击法提纯小胶质细胞的培养方法产量多,纯度高,为小胶质细胞在神经退行性疾病相关研究提供了基础。Objective: To establish a simple and efficient method for the separation and purification of primary microglia. Methods: The cerebral cortex tissues were collected from one-day-old neonatal SD rats,and cells were obtained through trypsin digestion.The single cell suspension was then cultured.After the cells were confluent on the bottom of the culture flask,the culture flask was vigorously tapped for 3 min,then centrifuged and the precipitate was collected and cultured again.The purity was identified by immunofluorescence. Results: The purity of microglia was 96.85%. Conclusion: Vigorously tapping the culture flask could achieve high yield and high purity of microglia,and it provided the foundation for the study of microglia in neurodegenerative diseases.

关 键 词：小胶质细胞 原代培养 纯化方法 神经退行性疾病 

分 类 号：R741[医药卫生—神经病学与精神病学]