不同浓度蒿甲醚对人涎腺腺样囊性癌细胞增殖、凋亡、侵袭、迁移的影响及其机制  

Effects of various concentrations of artemether on proliferation,apoptosis,invasion,and migration of salivary adenoid cystic carcinoma cells

作　　者：李帅 徐向华 刘继光[1] 刘学超 张洲铭 王彭议 LI Shuai;XU Xianghua;LIU Jiguang;LIU Xuechao;ZHANG Zhouming;WANG Pengyi(Jiamusi University School of Stomatology, Jiamusi 154003, China)

机构地区：[1]佳木斯大学口腔医学院,黑龙江佳木斯154003 [2]山东省妇幼保健院 [3]山东大学附属省立医院

出　　处：《山东医药》2019年第12期14-17,共4页Shandong Medical Journal

基　　金：山东省医药卫生科技发展计划(2017WSB03005)

摘　　要：目的观察不同浓度的蒿甲醚(ART)对人涎腺腺样囊性癌(SACC)细胞增殖、凋亡、侵袭、迁移的影响,并探讨其机制。方法将SACC细胞系ACC-2分为4组,分别加入125、250、500μg/m L的ART或等量0. 1%DMSO培养液进行培养,记为125μg/m L组、250μg/m L组、500μg/m L组、对照组。采用CCK-8法测算各组细胞增殖率,采用流式细胞术Annexin V-FITC/PI双染法测算各组细胞凋亡率,采用Transwell侵袭实验检测各组细胞侵袭能力,采用Transwell迁移实验检测各组细胞迁移能力,采用Western blotting法检测各组细胞HMGB1蛋白。结果培养0、24、48、72、96 h时,125μg/m L组细胞增殖率分别为100. 7%±8. 7%、77. 5%±5. 2%、61. 0%±7. 7%、50. 6%±3. 3%、39. 0%±2. 6%,250μg/m L组细胞增殖率分别为99. 5%±7. 3%、63. 3%±3. 6%、50. 4%±3. 4%、38. 1%±2. 7%、25. 5%±2. 3%,500μg/m L组细胞增殖率分别为101. 5%±2. 4%、56. 0%±2. 9%、38. 2%±3. 7%、28. 9%±2. 3%、11. 0%±2. 4%,对照组细胞增殖率均为100. 0%,各组培养24、48、72、96 h时细胞增殖率相比,P均<0. 01。125μg/m L组、250μg/m L组、500μg/m L组、对照组细胞凋亡率分别为21. 98%±0. 44%、34. 76%±1. 74%、49. 94%±1. 43%、2. 30%±0. 17%,侵袭细胞数目分别为(61±3)、(39±3)、(15±1)、(99±8)个,迁移细胞数分别为(93±13)、(69±3)、(30±3)、(164±16)个,细胞HMGB1蛋白相对表达水平为0. 798±0. 064、0. 667±0. 079、0. 424±0. 019、0. 887±0. 045,上述各指标组间相比,P均<0. 01。结论 125、250、500μg/m L的ART对SACC细胞增殖、凋亡、侵袭、迁移均可发挥调节作用,ART可能通过下调HMGB1蛋白的表达调控SACC细胞的增殖、凋亡、侵袭和迁移。Objective To observe the effects of various concentrations of artemether( ART) on the proliferation,apoptosis,invasion,and migration of salivary adenoid cystic carcinoma( SACC) cells and to explore the underlying mechanisms. Methods SACC cells ACC-2 were divided into four groups,and cultured with 125,250,500 μg/m L ART and an equal amount of 0. 1% DMSO medium,which were taken as the 125 μg/m L group,250 μg/m L group,500 μg/m L group,and control group. The rate of cell proliferation was detected by CCK-8. The Annexin V-FITC/PI double staining method was carried out to examine the apoptosis. Then,the invasive ability was quantified by Transwell invasion test. Transwell migration test was used to detect the migration ability. Further,the expression of HMGB1 protein was analyzed by Western blotting. Results At 0,24,48,72,and 96 h,the rates of cell proliferation in the 125 μg/m L group were 100. 7%±8. 7%,77. 5%± 5. 2%,61. 0%± 7. 7%,50. 6%± 3. 3%,and 39. 0%± 2. 6%;the rates of cell proliferation in the 250 μg/m L group were 99. 5%± 7. 3%,63. 3%± 3. 6%,50. 4%± 3. 4%,38. 1%± 2. 7%,and 25. 5%± 2. 3%;the rates of cell proliferation in the 500 μg/m L group were 101. 5%± 2. 4%,56. 0%± 2. 9%,38. 2%± 3. 7%,28. 9%±2. 3%,and 11. 0%± 2. 4%;the rates of cell proliferation in the control group were 100. 0%;significant differences were found in the rates of cell proliferation between groups at different time points( all P < 0. 01). The apoptotic rates in the 125μg/m L,250 μg/m L,500 μg/m L groups and control group were 21. 98%± 0. 44%,34. 76%± 1. 74%,49. 94%±1. 43%,and 2. 30%± 0. 17%,respectively. The number of invasive cells were 61 ± 3,39 ± 3,15 ± 1,and 99 ± 8,respectively;the number of migration cells were 93 ± 13,69 ± 3,30 ± 3,and 164 ± 16,respectively;furthermore,the relative expression levels of high mobility group box-1( HMGB1) protein were 0. 798 ± 0. 064,0. 667 ± 0. 079,0. 424 ±0. 019,and 0. 887 ± 0. 045,respectively;significant difference was found between groups( all P < 0. 01). Co

关 键 词：青蒿素衍生物 蒿甲醚 腺样囊性癌 涎腺腺样囊性癌 高迁移蛋白-1 细胞增殖 细胞凋亡 细胞侵袭 细胞迁移 

分 类 号：R739.8[医药卫生—肿瘤]