淫羊翟昔对钛颗粒诱导溶骨模型小鼠miR-21-5p表达的影响  

Effect of Icariin on Expression of miR-21-5p in Osteolysis Induced by Titanium Particles

作　　者：连峰[1] 赵承斌[1] 曲敬[1] 廉永云[1] 崔勇[1] 吴立文[1] 陈会超[1] LIAN Feng;ZHAO Cheng-bin;QU Jing(Department of Orthopedics,the Fourth Affiliated Hospital of Harbin Medical University,Harbin 510001,Heilongjiang)

机构地区：[1]哈尔滨医科大学附属第四医院骨科,黑龙江哈尔滨510001

出　　处：《医学临床研究》2019年第3期417-419,共3页Journal of Clinical Research

摘　　要：【目的】探讨淫羊餐甘对钛颗粒诱导骨溶解模型小鼠miR-21-5p表达的影响。【方法】6周龄雄性Balb/c小鼠随机平均分为假手术组、钛颗粒组和淫羊蒼甘组。淫羊餐甘组采用钛颗粒诱导小鼠颅骨骨溶解模型,并于术后每天给予300mg/kg的淫羊蹩甘.持续灌胃14do采用RealtimePCR技术分别检测小鼠手术前、给药后3d、7d、14d血清miR-21-5p的相对表达量及给药后14d颅骨组织miR-21-5p的相对表达量。【结果】钛颗粒诱导骨溶解手术前.各组小鼠血清miR-21-5p的相对表达量无差异。手术后,与假手术组相比,钛颗粒组小鼠血清与颅骨组织miR-21-5p的相对表达量显著降低(P<0.01);与钛颗粒组相比,淫羊餐甘组小鼠血清与颅骨组织miR-21-5p的相对表达量显著升高(P<0.01)。【结论】淫羊餐昔能够显著促进钛颗粒诱导骨溶解模型小鼠血清与颅骨组织miR-21-5p的表达.提示miR-21-5p可以作为骨溶解治疗的靶点。[Objective] To investigate the effect of icariin(ICA)on the expression of miR-21-5p in osteolysis induced by titanium particles.[Methods]Six-week-old male Balb/c mice were randomly divided into sham operation group(SOP group),the titanium particle group(TiP group)and the icarin group(ICA group).In the ICA Group,titanium particles were used to induce osteolysis in mice,and 300 mg/kg of icariin was given daily for 14 days by intragastric administration after the operation.Real-time PCR was used to detect the relative expression of miR-21-5p in mice serum before the operation and 3 days,7 days,14 days after administration of icariin.The relative expression of miR-21-5p in skull of mice was detected by Real-time PCR at 14 daysafter icarin administration.[Results]There was no difference in the relative expression of serum miR-21-5p in each group before osteolysis-induced by titanium particles.Compared to the SOP Group,the relative expression of miR-21-5p in serum and skull from the TiP Group was significantly decreased after the operation(P<0.01).Compared to the TiP Group,the relative expression of miR-21-5p in serum and skull of the ICA group was significantly increased(P<0.01).[Conclusion]Icarin can significantly promote the expression of miR21-5p in serum and skull of mice after osteolysis induced by titanium particles.These results suggest that mir21-5p can be used as a target for osteolysis therapy.

关 键 词：小鼠 近交BALBC/损伤 药用制剂 

分 类 号：R-332[医药卫生]