监测BCAR1阳性循环肿瘤细胞在早期肺腺癌术后随访中的应用  

The application of detecting BCARI (+)- circulating tumor cells in postoperative monitoring of early stage lung adenocarcinoma

作　　者：蔡云婷[1] 钱凯[1] 冯涌耕 王如文[1] 周景海[1] 谭群友[1] 邓波[1] Cai Yunting;Qian Kai;Feng Yonggeng;Wang Rawed;Zhou Jinghai;Tan Qunyou;Deng Bo(Department of Thoracic Surgery, Institute of Surgery Research , Doping Hospital, Army Medical University, Chongqing 400042 , China)

机构地区：[1]中国人民解放军陆军军医大学大坪医院野战外科研究所胸外科,重庆400042

出　　处：《中华胸心血管外科杂志》2019年第3期163-166,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

摘　　要：目的探索在早期肺腺癌患者中监测BCARI阳性循环肿瘤细胞BCAR1(+)-CTCs的临床意义。方法2015年11月至2018年1月,共纳入48例I期肺腺癌患者,采用CanPatrolTM技术及RNA原位杂交检测患者外周血中CTCs及BCAR1基因表达情况。结果41例检测出CTCs,检出率85.4%,30例检出BCARI(+)-CTCs,检出率62.5%0BCAR1(+)-CTCs和CTCs总数量显著正相关。BCAR1(+)更易出现在发生过上皮间质转化的CTCs,即M-CTCS及E&M-CTCS中。复发3例术后BCARI(+)-CTCs显著增高。45例病情稳定的患者中,19例术后持续监测CTCs,其中12例总CTCs及BCAR1(+)-CTCs均显著降低或保持稳定;7例总CTCs升高,其中2例BCARI(+)-CTCs保持稳定,1例降低,4例BCAR1(+)-CTCs增高者继续密切随访。结论动态监测BCAR1(+)-CTCs有助于预测早期肺腺癌复发或转移。Objective To explore the clinical significance regarding monitoring circulating tumor cells in early stage lung adenocarcinoma. Methods From November 2015 to January 2018 , 48 patients with stage I lung adenocarcinoma were included in the study. BCARI expression in CTCs in peripheral blood were detected by using CanPatrolTM and RNA in situ hybridization detection. Results Among the 48 cases, CTCs and BCARI (+)- CTCs were detected in 41 cases(85.4%) and 30 cases(62. 5%), respectively. Number of BCARI (+)-CTCs seemed to be significantly positively related to that of CTCs.BCARI (+)-CTCs were more likely to appear in the M - CTCS and E&M - CTCS. BCARI (+)-CTCs remarkably increased in three relapsed cases. Furthermore, there were 19 stable cases who had postoperative CTCs data:( 1) in 12 patients, either CTCs or BCARI (+)- CTCs were significantly reduced or remained stable;(2) in 7 cases, CTCs increased, but BCARI (+)-CTCs remained stable in 2 cases, reduced in 1 case, and the other 4 cases underwent close follow-up. Conclusion Evaluation of BCARI (+)- CTCs possibly can be contributive to prediction of early lung adenocarcinoma recurrence or metastasis.

关 键 词：循环肿瘤细胞 乳腺癌抗雌激素药物耐药蛋白1 上皮间质转化 肺腺癌 

分 类 号：R734.2[医药卫生—肿瘤]