NIR-II ?uorescence in vivo confocal microscopy with aggregation-induced emission dots  

作　　者：Wenbin Yu Bing Guo Hequn Zhang Jing Zhou Xiaoming Yu Liang Zhu Dingwei Xue Wen Liu Xianhe Sun Jun Qian 

机构地区：[1]State Key Laboratory of Modern Optical Instrumentations, Centre for Optical and Electromagnetic Research, College of Optical Science and Engineering, Zhejiang University [2]Department of Chemical and Biomolecular Engineering, National University of Singapore [3]Department of Urology, Sir Run-Run Shaw Hospital College of Medicine, Innovation Center for Minimally Invasive Technique and Device, Zhejiang University [4]Interdisciplinary Institute of Neuroscience and Technology (ZIINT), Zhejiang University [5]Joint Research Laboratory of Optics of Zhejiang, Normal University and Zhejiang University, Zhejiang Normal University

出　　处：《Science Bulletin》2019年第6期410-416,共7页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(61735016);Zhejiang Provincial Natural Science Foundation of China(LR17F050001)

摘　　要：Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection(e.g.,via InGaAs camera),which has high temporal resolution but limited spatial resolution due to out-of-focus signals.Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues.In this presented work,a NIR-II fluorescence confocal microscopic system was setup.By using a kind of aggregation-induced emission(AIE)dots as NIR-II fluorescent probes,800 lm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained,and the spatial resolution at 700 lm depth could reach 8.78 lm.Moreover,the time-correlated single photon counting(TCSPC)technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy,and in vivo confocal NIR-II fluorescence lifetime microscopic imaging(FLIM)of mouse cerebral vasculature was successfully realized.Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection(e.g.,via InGaAs camera),which has high temporal resolution but limited spatial resolution due to out-of-focus signals.Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues.In this presented work,a NIR-II fluorescence confocal microscopic system was setup.By using a kind of aggregation-induced emission(AIE)dots as NIR-II fluorescent probes,800 lm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained,and the spatial resolution at 700 lm depth could reach 8.78 lm.Moreover,the time-correlated single photon counting(TCSPC)technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy,and in vivo confocal NIR-II fluorescence lifetime microscopic imaging(FLIM)of mouse cerebral vasculature was successfully realized.

关 键 词：Confocal microscopy NIR-II fluorescence AIE DOTS In vivo CEREBROVASCULAR IMAGING TCSPC FLIM IMAGING 

分 类 号：N[自然科学总论]