甜菜红素合成相关基因不同时期叶片表达情况分析  

Expression of Betacyanin Synthesis Related Genes in Beet at Different Stages

作　　者：鹿尧 姚磊 代翠红 李新玲[4] 张延明[4] LU Yao;YAO Lei;DAI Cui-hong;LI Xin-ling;ZHANG Yan-ming(National Research Center of Soybean Engineering and Technology,Northeast Agricultural University,Harbin 150028,China;Heilongjiang Green Food Science Research Institute,Harbin 150028,China;School of Chemistry and Chemical Engineering,Harbin Institute of Technology,Heilongjiang,150086,China;School of Life Science and Technology,Harbin normal university,Harbin Heilongjiang 150020,China)

机构地区：[1]东北农业大学国家大豆工程技术研究中心,哈尔滨150028 [2]黑龙江省绿色食品科学研究院,哈尔滨150028 [3]哈尔滨工业大学化工与化学学院,黑龙江哈尔滨150086 [4]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150020

出　　处：《中国甜菜糖业》2019年第1期12-16,25,共6页China Beet & Sugar

基　　金：国家自然科学基金(31501360;31371685)。

摘　　要：本实验以红甜菜叶片为材料,采用Real-time PCR技术,并结合2-△△Ct法处理数据,对不同生长时期甜菜叶片中的红色素合成相关酶基因的相对表达情况进行了分析。结果表明:酪氨酸酶基因在五对真叶期以前相对表达量较高,说明其可能在早期甜菜叶片中红色素的合成起着一定作用,其前期的高表达有助于红色素在叶片中的积累;葡糖基转移酶基因在甜菜生长的各个时期都发挥着作用,而且各个生长时期其相对表达量差异较小,这说明该基因在整个甜菜生长期都在稳定调控红色素合成;4,5-DOPA-双加氧酶基因在叶片生长完成后,其基本不表达。研究明确了甜菜红色素合成相关基因在不同时期相对表达量情况,实验结果为研究红色素在甜菜叶中不同时期合成代谢提供理论基础和依据。In this experiment,the red beet leaves were used as materials,and the expression of betacyanin synthesis related genes was analyzed by using Real-time PCR and 2^-△△Ct method.The results showed that the expression of tyrosinase gene was higher before the five pairs of true leaf stage.It indicated that it might play a role in the synthesis of betacyanin in beet leaves at the early stages of growth,and its high expression at the early stage helps the store of betacyanin in the leaves.The glucosyltransferase gene plays a role in the growth of sugar beet,and the relative expression difference is small in each growth period,which indicates that the gene stably regulates betacyanin synthesis throughout the sugar beet growth period.When all the leaves have grown up,the 4,5-DOPA-dualoxygenase gene is barely expressed.The study clarified the expression of the genes related to betacyanin synthesis in different periods.The experimental results provide a theoretical basis for studying the anabolism of betacyanin at different stages of sugar beet leaves.

关 键 词：甜菜 红色素 基因表达量分析 

分 类 号：S566.3[农业科学—作物学]