Aldosterone may induce visfatin expression via the GR-ERK1/2 signal pathway in 3T3-L1 adipocytes  

作　　者：Junhua Ma Xuemei Zhao Jichen Zhang Fei Sun Jiancan Lu Hongling Zhu Mingjun Gu Jingnan Wang Xiangqi Li 

机构地区：[1]Department of Endocrinology,Shanghai Gongli Hospital,the Second Military Medical University,Shanghai 200135,China

出　　处：《Acta Biochimica et Biophysica Sinica》2019年第3期331-334,共4页生物化学与生物物理学报（英文版）

基　　金：the grants from the National Natural Science Foundation of China(No.81500655);the Postdoctoral Cultivation Fund of Shanghai Pudong New Area Gongli Hospital(No.GLBH 2017005);the Talent Project of Shanghai Pudong New Area Gongli Hospital(No.GLRb2017-01);the Key Discipline Program of Pudong New Area Health System of Shanghai(No.PWZxk2017-07);the Special Disease Program of Pudong New Area Health System of Shanghai(No.PWZzb2017-22);the Shanghai Health and Family Planning Commission(No.201540393).

摘　　要：The main function of aldosterone is to regulate blood pressure and electrolytic balance;however,recent studies have also focused on the effect of aldosterone on glucose metabolism especially in adipose tissue.Aldosterone was found to inhibit insulin-induced glucose uptake in 3T3-L1 adipocytes [1] and aldosterone deficiency was found to prevent high-fat-feeding-induced hyperglycemia and adipocyte dysfunction in mice [2].Visfatin,an adipokine discovered by the team of Fukuhara,is mainly released by adipose tissue [3].Treatment of 3T3-L1 adipocytes and L6 myocytes with visfatin increased basal glucose uptake,and glucose release was suppressed in H4IIEC3 hepatocytes after visfatin treatment [3].It has also been suggested that visfatin may act as a proinflammatory cytokine [4] and produce chronic inflammation in adipocytes [5].Eyileten et al.[6] reported that treatment with angiotensin-converting enzyme inhibitor,which can reduce plasma aldosterone concentration,decreased circulating visfatin level in diabetic nephropathy patients.These findings suggest a possible interaction between the renin–angiotensin–aldosterone system(RAAS)and visfatin.Considering the important roles of aldosterone and visfatin and the association between RAAS and visfatin,we hypothesize that there may be a direct regulatory interaction between aldosterone and visfatin.To test this hypothesis,3T3-L1 adipocytes were used as the cell model and the expression of visfatin was measured by qRT-PCR and western blot analysis.

关 键 词：GR-ERK1/2 3T3-L1 ADIPOCYTES renin-angiotensin-aldosterone system(RAAS) 

分 类 号：Q[生物学]