A competitive microcystin-LR immunosensor based on Au NPs@metal-organic framework(MIL-101)  

作　　者：Kunlei Zhang Kun Dai Ruyan Bai Yuchan Ma Yan Deng Delei Li Xi Zhang Rong Hu Yunhui Yang 

机构地区：[1]College of Chemistry and Chemical Engineering, Yunnan Normal University

出　　处：《Chinese Chemical Letters》2019年第3期664-667,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos. 21165023,21465026, 21765026, 21605130);the National Key Scientific Program of China(Nos. 2011CB911000,01100205020503104)

摘　　要：An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks(MOFs) material based on trivalent chromium skeleton were synthesized by hydrothermal synthesis method, and loaded with Au nanoparticles(Au NPs) to prepare Au NPs@MIL-101 composite materials which were used as a marker to label anti microcystin-LR(Anti-MC-LR). The composite materials have strong catalytic properties to the oxidation of ascorbic acid. Anti-MC-LR was immobilized on glassy carbon electrode surface using electrodeposition graphene oxide(GO) as an immobilization matrix to construct a competitive microcystin-LR immunosensor. The electrochemical immunosensor display linear relationship in the range of 0.05 ng/mL-75 μg/mL with linear correlation coefficient of 0.9951 and detection limit of 0.02 ng/mL(S/N = 3). This sensor was used to detect microcystin-LR in the water sample. The recovery was 102.43%,which is satisfied. The good testing results indicate the sensor has a great prospect in practical application.An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks(MOFs) material based on trivalent chromium skeleton were synthesized by hydrothermal synthesis method, and loaded with Au nanoparticles(Au NPs) to prepare Au NPs@MIL-101 composite materials which were used as a marker to label anti microcystin-LR(Anti-MC-LR). The composite materials have strong catalytic properties to the oxidation of ascorbic acid. Anti-MC-LR was immobilized on glassy carbon electrode surface using electrodeposition graphene oxide(GO) as an immobilization matrix to construct a competitive microcystin-LR immunosensor. The electrochemical immunosensor display linear relationship in the range of 0.05 ng/mL-75 μg/mL with linear correlation coefficient of 0.9951 and detection limit of 0.02 ng/mL(S/N = 3). This sensor was used to detect microcystin-LR in the water sample. The recovery was 102.43%,which is satisfied. The good testing results indicate the sensor has a great prospect in practical application.

关 键 词：MICROCYSTIN-LR METAL-ORGANIC frameworks MIL-101 COMPETITIVE ELECTROCHEMICAL IMMUNOSENSOR 

分 类 号：O6[理学—化学]