ABR处理综合印染废水中细菌群落结构研究  

Study on Microbial Communities in an Anaerobic Baffled Reactor Treating Printing and Dyeing Wastewater

作　　者：邢立群 王力超 杨立业 XING Liqun;WANG Lichao;YANG Liye(Yancheng Academy of Environmental Protection Technology and Engineering, Nanjing University, Yancheng 224000, China;Shouguang Vocational Education Center School, Weifang 26270(), China)

机构地区：[1]南京大学盐城环保技术与工程研究院,江苏盐城224000 [2]寿光市职业教育中心学校,山东潍坊262700

出　　处：《环境科学与技术》2019年第3期1-8,共8页Environmental Science & Technology

基　　金：国家水体污染控制与治理科技重大专项(2017ZX07602-004)

摘　　要：印染废水是公认的难降解工业废水之一,以细菌菌群为主的厌氧生物污泥决定印染废水的脱色和解毒效果,但是基于厌氧折流板反应器(ABR)处理综合印染废水工程污泥细菌群落结构的研究鲜见报道。该文基于Miseq高通量测序分析技术,对长期稳定运行的ABR处理单元6个隔室的微生物菌群分布进行研究。结果表明,ABR处理单元中微生物菌群多样性较为丰富,且各隔室间存在较大差异。前5个隔室中优势细菌门类为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、螺旋体门(Spirochaetae)和厚壁菌门(Firmicutes),而第6隔室为变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)和互养菌门(Synergistetes);主要属为螺旋体属(Spirochaetes_norank)、陶厄氏菌属(Thauera)、未命名菌属(vadinBC27_wastewater-sludge_group和Sh765B-TzT-29_norank)、长绳菌属(Longilinea)、副球菌属(Paracoccus)、利恩氏热杆菌属(Thermovirga)、丛毛单胞菌属(Comamonas)和假单胞菌属(Pseudomonas),大多数属与印染废水的脱色和降解过程有关。该结果为综合印染废水污染物的去除机制和运营提供了理论基础。Printing and dyeing wastewater is recognized as one of refractory industrial wastewater. The bacterial flora in anaerobic biological sludge mainly contributed to decolorization and detoxification of printing and dyeing wastewater. It is rarely reported the bacteria community structure in anaerobic baffled reactor (ABR) treating the comprehensive dyeing wastewater based on actual operation project. The distributions of microbial communities in six ABR compartments of stable operation for a long-term were investigated using Illumine Miseq sequencing analysis technology. Results showed that the diversity of microbial flora in ABR was relatively rich, and there were great differences among different compartments. The dominant bacteria in the first five ABR compartments were Proteobacteria, Bacteroidetes, Spirochaetae and Firmicutes, respectively;while dominant bacteria in the sixth compartment were Proteobacteria, Chloroflexi and Synergistetes, respectively. The dominant genera were Spirochaetes_norank, Thauera, vadin BC27_wastewater-sludge_group, Sh765B-TzT-29_norank, Longilinea, Comamonas and Pseudomonas, respectively, which were related to the decolorization and degradation process of printing and dyeing wastewater. These results might be helpful for the removal mechanism and stable operation in printing and dyeing wastewater.

关 键 词：印染废水 厌氧折流板反应器 细菌群落 Miseq高通量测序 

分 类 号：X172[环境科学与工程—环境科学]