Extending the operational lifetimes of all-direct electron transfer enzymatic biofuel cells by magnetically assembling and exchanging the active biocatalyst layers on stationary electrodes  

作　　者：Katharina Herkendell Andreas Stemmer Ran Tel-Vered 

机构地区：[1]Saumerstrasse 4, Nanotechnology Group, D-MAVT, ETH Zurich, Ruschlikon CH-8803, Switzerland

出　　处：《Nano Research》2019年第4期767-775,共9页纳米研究（英文版）

摘　　要：Enzymatic biofuel cells promise green power generation from a variety of natural resources, yet these systems all suffer from time-dependent degradati on effects, in particular progress! ng inactivation of enzymes, which severely limit the operati on al lifetimes of such power sources. To extend operational lifetimes, we introduce a method to magnetically exchange exhausted enzymes for fresh ones. To this end, anodic and cathodic enzymes or enzyme cascades are immobilized on carbon coated magnetic nanoparticles. Under the action of suitable magnetic field gradie nts, these nano particles are assembled on the respective stati onary electrodes, or released from the electrodes for collection and subsequent excha nge. We dem on strate this method on a fructose/oxygen con suming biofuel cell emplo ying fructose dehydroge nase and bilirubin oxidase as well as on anodic and cathodic cascades employing fructose dehydrogenase/invertase and bilirubin oxidase/catalase, respectively. The en zyme-modified nan oparticles support direct electro n transfer bioelectrocatalytic curre nts by wiring the redox active cofactors to the carbonaceous coating and from there to the electrode surfaces. The facile injection, assembly, and removal of enzyme-modified magnetic nan oparticles along with fuel solutio n provides a promisi ng approach to exte nd the operati on al lifetime of enzymatic biofuel cells without the need for exchanging entire systems including chambers and electrodes.

关 键 词：direct electron transfer magnetic nanoparticle ENZYMATIC BIOFUEL cell RECHARGE lifetime ENZYME cascade 

分 类 号：O4[理学—物理]