北细辛苗在不同生长阶段与成熟期的主要化学成分分析  

Analysis of Main Components in Asarum heterotropoides var. mandshuricum Seedling at Different Growth Periods and Maturation Period

作　　者：陆亚男[1] 田晓清[1] 周晓云[2] 乔玉宝 唐莹莹 马丽艳[1] 樊成奇[1] LU Ya-nan;TIAN Xiao-qing;ZHOU Xiao-yun;QIAO Yu-bao;TANG Ying-ying;MA Li-yan;FAN Cheng-qi(Key Laboratory of Oceanic and Polar Fisheries ,Ministry of Agriculture,East China SeaFisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090,China;Jilin Hospital of Integrated Traditional Chinese and Western Medicine in Jilin Province, Jilin 132010,China;Shanghai Ocean University, Shanghai 201306,China)

机构地区：[1]中国水产科学研究院东海水产研究所农业部远洋与极地渔业创新重点实验室,上海200090 [2]吉林省吉林中西医结合医院,吉林吉林132010 [3]上海海洋大学,上海201306

出　　处：《中国实验方剂学杂志》2019年第9期159-165,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家基本药物所需中药材种子种苗繁育基地建设项目

摘　　要：目的:研究北细辛苗在生长过程中主要化学成分的变化规律。方法:分别采集生长1周和2周的北细辛苗以及生长3个月的北细辛植株,均分成地上(茎、叶)与地下部分(根),采用HPLC-TOF-MS对其次生代谢产物进行定性鉴别,以细辛脂素作为指标成分进行定量鉴别。结果:从北细辛地下部分共鉴定6个已知化合物,分别为α-细辛醚(1),N-isobutyl-2,4,8,10-dodecatetraenamide(2),9-methoxyaristolactamⅣ(3),细辛脂素(4),caulesnarinside(6)和chalcononaringenin 2’,4’-di-O-β-Dglucopyranoside(7),峰面积数值表明其含量随着生长时间的增长逐渐增加;从北细辛地上部分共鉴定出4个已知化合物,分别为N-isobutyl-2,4,8,10-dodecatetraenamide(2),caulesauroneside(5),caulesnarinside(6)或chalcononaringenin 2’,4’-di-O-β-Dglucopyranoside(7)和peonidin 3-caffeoylgentiobioside(8)。仅在生长3个月的北细辛地下部分鉴定出了细辛脂素,其质量分数155.4μg·g^(-1)。结论:北细辛地上与地下部分次生代谢产物种类与含量存在较大差异;在北细辛苗的不同生长阶段,相同部位的次生代谢产物的种类和含量也存在较大差异,其主要成分的含量基本呈现随生长时间增加而增加的趋势。Objective: To analyze the change rule of main chemical components in Asarum heterotropoides var. mandshuricum seedling during the growing process. Method: Whole seedling samples( one week and two weeks) and the mature plant( three months) of A. heterotropoides var. mandshuricum were collected and every sample was divided to aerial part( stems and leaves) and underground part( roots). The secondary metabolites were qualitatively identified by HPLC-TOF-MS and the quantitative identification was carried out at the same time with asarinin as index component. Result: A total of 6 known compounds were identified from the underground part of A. heterotropoides var. mandshuricum as α-asarone( 1), N-isobutyl-2,4,8,10-dodecatetraenamide( 2),9-methoxyaristolactam Ⅳ( 3),asarinin( 4),caulesnarinside( 6) and chalcononaringenin 2’,4’-di-O-β-D-glucopyranoside( 7), respectively, the peak area values showed that the contents of these compounds increased gradually with the growth time. A total of 4 known compounds were identified from the aerial part of this herb as N-isobutyl-2, 4, 8, 10-dodecatetraenamide( 2), caulesauroneside( 5), caulesnarinside( 6) or chalcononaringenin 2’,4’-di-O-β-D-glucopyranoside( 7) and peonidin 3-caffeoylgentiobioside( 8). Asarinin was identified only in the underground part of mature plant,its content was 155. 4 μg·g^(-1). Conclusion: The species and contents of secondary metabolites are quite different in the aerial and underground parts of A.heterotropoides var. mandshuricum. At different growth stages of A. heterotropoides var. mandshuricum seedling,the types and contents of secondary metabolites in the same site are also different, while the contents of main components show an increasing trend with the growth time.

关 键 词：北细辛 种苗 生长阶段 地上部分 地下部分 次生代谢产物 细辛脂素 

分 类 号：R22[医药卫生—中医基础理论] R931[医药卫生—中医学]