鲜天麻提取物对小鼠肠道菌群结构的影响  

Effect of fresh Gastrodia elata on gut microbiota in mice

作　　者：华中一 李洪梅[2] 孙建辉[2] 霍海如[2] 李小芹[2] 黄璐琦[1] 袁媛[1] HUA Zhong-yi;LI Hong-mei;SUN Jian-hui;HUO Hai-ru;LI Xiao-qin;HUANG Lu-qi;YUAN Yuan(National Resource Center for Chinese Materia Medical,China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药资源中心,北京100700 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2019年第5期1004-1009,共6页China Journal of Chinese Materia Medica

基　　金：中央级公益性科研院所基本科研业务费专项(ZZ10-008);中央本级重大增减支项目(2060302)

摘　　要：天麻Gastrodia elata Bl.作为传统中药,具有息风止痉,平抑肝阳,祛风通络的功效,常用于治疗头痛、偏头痛等神经系统疾病,已被提议加入"按照传统既是食品又是中药材物质的目录"。临床常用干天麻的药效及作用机制已有相应的研究基础。而鲜天麻作为保健药膳的常用原料目前还缺乏研究。近年来饮食-肠道菌群-宿主的相互作用,及其对维护人体健康的影响已引起关注。目前已发现许多药食两用物质都可以通过调节肠道菌群维护人体健康。该研究利用MiSeq平台对服用鲜天麻提取物的小鼠盲肠内容物进行16S rRNA测序,探究鲜天麻提取物对小鼠肠道菌群结构的影响。结果表明,天麻提取物可显著提高小鼠肠道菌的益生菌属如Ruminiclostridium,Butyricicoccus,Parvibacter的丰度;而致病菌和条件致病菌属如Escherichia/Shigella,Parasutterella等丰度下降,说明长期服用鲜天麻对小鼠肠道菌群具有一定的调节作用,其中低剂量鲜天麻提取物能够显著调节肠道菌群结构。该研究结果揭示了肠道菌群可能是天麻提取物新的作用靶点。Gastrodia elata B1.,a traditional Chinese medicine,was frequently applied as a cure for headache or migraine. Its effects include suppressing hyperactive liver,calming endogenous wind,dredging collateralsand relieving spasm. There has been a proportion that G. elata should be added to The List of Substances That Are Traditionally Both Food and Chinese Medicinal Materials. The dry G. elata was commonly used in clinic,which have some fundamental study on efficacy and mechanism. However,fresh G. elata,which was added to herbal cuisine very often,lacks corresponding research. The interaction of diet,microbiota and human is a hot issue and lots of scholars are focusing on it. This research sequenced the 16 S rRNA of mouse cecal contents on Mi Seq platform to understand the effect of taking fresh G. elata. As the results showing,multiple probiotics grew after taking fresh G. elata extract,including Ruminiclostridium,Butyricicoccus,and Parvibacter. To contrast,some pathogens or potential pathogens,such as Escherichia/Shigella,Parasutterella,decreased. This manifests that fresh G. elata performs a positive regulation on mouse gut microbiota,especially the low-dose fresh G. elata extraction could restructure the microbiota apparently. Our result reveals that microbiota might be a new target for G. elata extract and provides an important basis for further research on the interaction between gut microbiota and pharmacological activity of G. elata.

关 键 词：天麻 药食两用 肠道菌群 益生菌 代谢疾病 

分 类 号：R285.5[医药卫生—中药学]