微胶囊双水相法提取罗汉松挥发油及成分分析  

Extraction of Volatile Oil from Podocarpus macrophyllus by Microcapsule Aqueous Two-phase System and Its Composition Analysis

作　　者：王全泽 袁堂丰 瞿利民 宋科[1,2] 郭婕 WANG Quan-ze;YUAN Tang-feng;QU Li-min;SONG Ke;GUO Jie(Key Laboratory of Hunan Forest Product and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, Hunan, China;Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha410428, Hunan, China)

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000 [2]湖南省植物功能成分利用协同创新中心,湖南长沙410428

出　　处：《精细化工》2019年第4期684-690,729,共8页Fine Chemicals

基　　金：国家自然科学基金地区项目(31560105);湖南省自然科学基金项目(14JJ6036)~~

摘　　要：通过微胶囊和双水相萃取相结合的方法对罗汉松枝叶中总挥发油的提取工艺进行了考察。经过筛选,确认以?-环糊精-硫酸钠作为微胶囊双水相体系,在单因素实验基础上,通过四因素三水平响应面实验方法优化了罗汉松枝叶挥发油的提取条件。得到的最佳条件为:萃取时间36min,?-环糊精质量分数42%,硫酸钠质量分数15%,萃取温度33℃,在该条件下罗汉松枝叶中挥发油得率为1.369%。用气相色谱-质谱联用技术从挥发油中鉴定出化合物22种,其中,萜烯及其含氧衍生物18种,脂肪族化合物4种,主要物质为石竹烯、?-蒎烯和?-蒎烯。Total volatile oil from the branches and leaves of Podocarpus macrophyllus was extracted by the combination of microcapsule technology and aqueous two-phase extraction technology. After screening,β-cyclodextrin and sodium sulfate was selected as extraction agents in aqueous two-phase system. On the basis of single factor experiment, the extraction conditions for volatile oil from Podocarpus macrophyllus were optimized by four-factor and three-level response surface methodology. The optimum extraction conditions were obtained as follows: extraction time 36 min, mass fraction of β-cyclodextrin 42%, mass fraction of sodium sulfate 15%, and extraction temperature 33 ℃. Under these conditions, the yield of total volatile oil from Podocarpus macrophyllus was 1.369%. A total of 22 compounds were identified by GC-MS, among which, containing 18 kinds of terpenes and their oxygen-containing derivatives and 4 kinds of aliphatic compounds. The main components of total volatile oil were caryophyllene,α-pinene and β-pinene.

关 键 词：罗汉松 微胶囊 挥发油 GC-MS 提纯技术 

分 类 号：R284.2[医药卫生—中药学]