2型糖尿病进程中小鼠下丘脑室旁核和视上核nesfatin-1表达  

EXPRESSION OF NESFATIN-1 IN THE HYPOTHALAMIC PARAVENTRICULAR NUCLEUS AND THE SUPRAOPTIC NUCLEUS IN MICE DURING THE COURSE OF TYPE 2 DIABETES

作　　者：李晓玲 刘媛[2] 宋莉敏 董静[2,3] LI Xiaoling;LIU Yuan;SONG Limin;DONG Jing(Department of Obestetrics and Gynecology, Qingdao University Medical College, Qingdao 266071, China)

机构地区：[1]青岛大学医学部,妇产科专业山东青岛2660711 [2]青岛大学基础医学院,特种医学专业山东青岛2660711 [3]青岛大学生理学教研室,山东青岛2660711

出　　处：《青岛大学学报（医学版）》2019年第2期192-196,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金项目(31872791;31371168)

摘　　要：目的探讨2型糖尿病进程中nesfatin-1在小鼠下丘脑室旁核和视上核中的表达。方法将雄性昆明小鼠随机分为对照组(普通饮食喂养)、高脂饮食组和糖尿病组。糖尿病组小鼠腹腔注射链脲佐菌素,并给予高脂饮食喂养诱导2型糖尿病模型。比较3组小鼠血糖水平、稳态模型的胰岛素抵抗指数(HOMA-IR)及摄食量。分别于造模成功后的2、4、6、8、10、12周,采用免疫荧光组织化学技术检测各组小鼠下丘脑室旁核和视上核组织中nesfatin-1表达。结果糖尿病组小鼠血糖较高脂饮食组和对照组明显增高,且出现明显的胰岛素抵抗,同时摄食量增多。糖尿病组小鼠室旁核和视上核nesfatin-1阳性神经元数量在造模后前6周逐渐增加,后6周逐渐降低,总体nesfatin-1表达水平低于高脂饮食组和对照组。结论 2型糖尿病小鼠下丘脑室旁核和视上核中的nesfatin-1表达水平降低,其表达变化呈前期上升、后期下降的趋势。Objective To investigate the expression of nesfain-1 in the hypothalamic paraventricular nucleus (PVN) and the supraoptic nucleus (SON) in mice during the course of type 2 diabetes. Methods Male Kunming mice were randomly divi- ded into control group with a normal diet, high-fat diet group, and diabetes group. The mice in the diabetes group were given intrape- ritoneal injection of streptozotocin and a high-fat diet to induce diabetes. The three groups were compared in terms of the level of blood glucose, homeostasis model assessment of insulin resistance (HOMA-IR), and food intake. At 2,4,6,8,10, and 12 weeks after the model was successfully established, immunofluorescence assay was used to measure the expression of nesfatin-1 in the PVN and the SON. Results Compared with the high-fat diet group and the control group, the diabetes group had significantly higher blood glucose level, HOMA-IR, and food intake. In the diabetes group, there was a gradual increase in the number of nesfatin-1-positive neurons in the PVN and the SON during the first 6 weeks, followed by a gradual reduction in the next 6 weeks, and the diabetes group generally had lower expression of nesfatin-1 than the high-fat diet group and the control group. Conclusion There is a reduction in the expression of nesfatin-1 in the hypothalamic PVN and the SON in mice with type 2 diabetes, and the expression of nesfatin-1 increases in the early stage and then decreases in the late stage.

关 键 词：NESFATIN-1 糖尿病 2型 下丘脑室旁核 视上核 小鼠 

分 类 号：R338.2[医药卫生—人体生理学]