5-羟色胺对油菜幼苗干旱的缓解效应  

Effect of exogenous 5-hydroxytryptamine on rape (Brassica napus L.) seedling under drought stress

作　　者：田姗姗 李继强 邹锡玲[1] 张学昆[1] 付桂萍[1] 吕艳 曾柳[1] 闫蕾 刘月[1] 钟燕[3] 杨静[3] 陈建军 马海清 程勇[1] TIAN Shan-shan;LI Ji-qiang;ZOU Xi-ling;ZHANG Xue-kun;FU Gui-ping;LYU Yan;ZENG Liu;YAN Lei;UU Yue;ZHONG Yan;YANG Jing;CHEN Jian-jun;MA Hai-qing;CHENG Yong(Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;Zhangye Academy of Forestry Science,Zhangye 734000,China;Hubei Vocational College of Bio - Technology,Wuhan 430070,China;Agricultural Technology Promotion Center in Qichun County,Qichun 435300,China;Oil Crop Station,Agricultural Bereau of Xishui County,Xishui 438200,China)

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]张掖市农业科学研究院,甘肃张掖734000 [3]湖北生物科技职业学院,湖北武汉430070 [4]蕲春县农业技术推广中心,湖北蕲春435300 [5]浠水县农业局油料作物推广站,湖北浠水438200

出　　处：《中国油料作物学报》2019年第2期192-198,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划项目(2017YFD0101700);中国农业科学院科技创新工程;湖北省农业科技创新中心;农业部油料作物生物学与遗传育种重点实验室开放课题(2017011)

摘　　要：为探讨5-羟色胺(5-HT,又名血清素)对油菜耐旱性的调节作用,以甘蓝型油菜品种中双11号为材料,通过水培试验,采用PEG6000 (polyethylene glycol)胁迫模拟干旱,分析了干旱条件下施加外源5-羟色胺对油菜幼苗生长、活性氧代谢及渗透调节物质的影响。结果表明,在干旱模拟胁迫下,油菜的生长受到抑制,过氧化氢(H_2O_2)、丙二醛(malondialdehyde,MDA)、脯氨酸(proline,Pro)以及可溶性糖(soluble sugar,SS)的含量明显增加,过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)和抗坏血酸过氧化物酶(ascorbate peroxidase,APX)的活性显著提高。在干旱模拟条件下施加外源5-羟色胺可以缓解干旱对油菜幼苗的不良影响,促进其生长。与单纯干旱模拟条件下的幼苗相比,添加外源5-羟色胺显著地提高了CAT、SOD、APX的活性,减少了过氧化氢和丙二醛的积累,同时渗透调节物质脯氨酸与可溶性糖含量显著增加。以上结果说明,外源5-羟色胺通过增加油菜幼苗的抗氧化酶活性来清除过多的过氧化氢和丙二醛,减轻膜脂过氧化反应,同时提高渗透调节物质含量从而缓解干旱胁迫对油菜幼苗的不利因素,促进油菜幼苗生物量积累,提高油菜幼苗的耐旱能力。5-hydroxytryptamine(5-HT,serotonin)is a naturally occurring compound in plants.To reveal the effects of 5-HT on rape(Brassica napus L.)seedlings under drought,effect of exogenous 5-HT on rape(Brassica napus L.)growth under PEG stress were investigated.The traits of seedling growth,active oxygen metabolism and osmotic adjustment substance were evaluated.Results showed that the simulative drought inhibited seedling growth,resulted in H2O2 and malondialdehyde(MDA)accumulation,increased proline(Pro)and soluble sugar(SS)content,and significantly enhanced the activity of catalase(CAT),superoxide dismutase(SOD)and ascorbate peroxidase(APX).Under the simulative drought condition,exogenous 5-HT alleviated the inhibi-tion on seedling growth.The activity of antioxidant enzymes(CAT,SOD and APX)was improved significantly by exogenous 5-HT,and the content of H2O2 and MDA was decreased.Results also showed that solute accumulation under the stress was significantly enhanced with exogenous 5-HT by increasing the content of Pro and SS.This study indicated that exogenous 5-HT could improve H2O2-scavenging capacity of rape seedlings by enhancing the activity of antioxidant enzymes and also alleviate osmotic stress by promoting the accumulation of osmoregulatory substances.Ultimately,exogenous 5-HT improved the biomass of rape seedlings under drought stress,and effectively alleviated drought stress in rape seedlings.

关 键 词：甘蓝型油菜 5-羟色胺 模拟干旱 幼苗 

分 类 号：S565.4[农业科学—作物学] S311