前房注射卡波姆建立大鼠高眼压模型的观察  

Observation of ocular hypertension model in rats induced by anterior chamber injection of carbomer suspension

作　　者：赵靖康 韩双羽 刘慧峰[2] 任媛[2] 王霞[2] 刘旭[2] 何媛[2] ZHAO Jing-Kang;HAN Shuang-Yu;LIU Hui-Feng;REN Yuan;WANG Xia;LIU Xu;HE Yuan(From the Xi’an Medical University,Xi’an 710068,Shaanxi Province,China;Department of Ophthalmology,the Second Affiliated Hospital of Xi’an Medical University,Xi’an 710038,Shaanxi Province,China)

机构地区：[1]西安医学院,陕西省西安市710068 [2]西安医学院第二附属医院眼科,陕西省西安市710038

出　　处：《眼科新进展》2019年第5期406-410,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助项目(编号:81770929)~~

摘　　要：目的前房注射卡波姆建立大鼠高眼压模型,观察卡波姆升眼压效果及对大鼠眼前节和视网膜的影响。方法随机选取30只SD大鼠,注射前3 d早晚测量基线眼压。右眼定为实验眼,左眼定为对照眼,右眼放出房水后将30μL的5 g·L^(-1)卡波姆混悬液注入前房,每日早10时、晚22时在大鼠清醒状态下测量眼压。每周进行双眼眼前节照相并对比。4周末处死26只大鼠(另4只持续观察眼压变化至注射后9周)并取双眼眼球行HE染色,观察实验眼与对照眼视网膜形态,对比视网膜厚度及房角形态。结果注射前,实验眼白天和夜间眼压分别为(11.10±0.90)mmHg(1 kPa=7.5 mmHg)和(11.92±1.07)mmHg,对照眼分别为(11.22±1.07)mmHg和(11.76±1.08)mmHg;实验眼与对照眼相比,白天、夜间眼压差异均无统计学意义(均为P>0.05);白天与夜间眼压相比,实验眼、对照眼差异均有统计学意义(均为P<0.05)。卡波姆在前房中呈现出弥散型和沉积型两种存在方式,弥散型和沉积型大鼠1周内眼压分别为(17.83±3.54)mmHg和(13.00±1.55)mmHg,两者相比差异具有统计学意义(P<0.05)。注射后第1天至第19天,实验眼与对照眼白天眼压相比差异均具有统计学意义(均为P<0.05);注射后第1天至第27天,实验眼与对照眼夜间眼压相比差异均具有统计学意义(均为P<0.05)。实验眼视网膜形态发生改变,注射后4周视网膜厚度为(254.70±21.80)μm,与对照眼的(346.73±24.63)μm相比,差异有统计学意义(P=0.00)。实验眼前房充满卡波姆及虹膜的混合成分,紧贴角膜内皮并延伸至房角,堵塞小梁网结构,正常虹膜形态消失;对照眼房角形态正常。结论前房注射卡波姆建立大鼠高眼压模型,可维持高眼压4周以上,昼夜眼压差异较为明显,夜间眼压较白天更高,4周后视网膜出现高眼压损伤后的表现。Objective To establish a rat model of elevated intraocular pressure (IOP) by injecting Carbomer suspension into the anterior chamber and observe the effect of carbomer on hypertension and the changes in anterior segment and retina. Methods Thirty Sprague Dawley (SD) rats were randomly selected and baseline IOP was measured 3 days before injection.The right eye was served as the experimental eye and the left as the control.After the aqueous humor was discharged,30 μL 5 g·L ^-1 Carbomer suspension was injected into the anterior chamber.The IOP was measured at 10 a.m.and 22 p.m.in the awake state of rats.Photography of the anterior segment was taken every week.After 4 weeks,26 rats were sacrificed and the eyeballs were enucleated for hematoxylin-eosin (HE) staining (Four of the 30 rats were observed to 9 weeks after Carbomer suspension injection).Comparison of the retinal thickness and anterior chamber angle was performed between experimental group and the control group under light microscope. Results Before injection,the IOP of the experimental eyes was (11.10±0.90)mmHg (1 kPa=7.5 mmHg) during daytime and (11.92±1.07)mmHg at night,while the control eyes was (11.22±1.07)mmHg and (11.76±1.08)mmHg,respectively.There was no significant difference in the IOP between the experimental eye and the control eye during the day and at night (all P >0.05),but it showed significant differences between daytime and night in experimental eyes and control eyes(all P <0.05).There were two kinds of Carbomer forms (diffuse and sedimentary form) in the anterior chamber.The 1-week mean IOP of diffuse and sedimentary form were (17.83±3.54)mmHg and (13.00±1.55)mmHg.There were significant differences in the mean IOP of daytime between the experimental eyes and the control eyes from the first day to the 19th day after injection (all P <0.05).There were significant differences in the mean night IOP between the experimental eyes and the control eyes from the 1st day to the 27th day after injection (all P <0.05).The morphology of retina

关 键 词：动物模型 高眼压 卡波姆 青光眼 大鼠 

分 类 号：R775[医药卫生—眼科]