Tie2基因对纳米金颗粒进入牙周组织的影响  

Effect of Tie2 gene on diffusion of gold nanoparticles into periodontal tissue

作　　者：李辰[1] 李许演 周敏 李卓权[3] 时函[4] LI Chen;LI Xu-yan;ZHOU Min;LI Zhuo-quan;SHI Han(Dept.of Endodontics,School & Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai 200072,China;Dept.of Periodontics,School & Hospital of Stomatology,TongjiUniversity,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai 200072,China;The Institute for Biomedical Engineering and Nanoscience,School of Medicine,Tongji University,Shanghai 200092,China;Dept.of Orthodontics,School & Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai 200072,China)

机构地区：[1]同济大学口腔医学院及附属口腔医院牙体牙髓病科上海市牙组织修复与再生工程技术研究中心,上海200072 [2]同济大学口腔医学院及附属口腔医院牙周病科上海市牙组织修复与再生工程技术研究中心,上海200072 [3]同济大学医学院生物医学工程与纳米科学研究院,上海200092 [4]同济大学口腔医学院及附属口腔医院口腔正畸科上海市牙组织修复与再生工程技术研究中心,上海200072

出　　处：《同济大学学报（医学版）》2019年第2期180-183,189,共5页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(81600855);上海市卫计委科研课题(20164Y0129)

摘　　要：目的研究Tie2基因表达对不同粒径纳米金颗粒(gold nanoparticles,GNPs)进入牙周组织及停留情况的影响。方法采用实时定量PCR和Western印迹法检测Tie2基因和蛋白在正常和转基因小鼠牙周组织中的表达;冰冻切片检测GFP在牙周组织中相对高表达的部位;小鼠尾静脉注射1 g/kg剂量20、40、60、80 nm粒径GNPs,应用ICP-AES技术检测牙周组织Au含量。结果转基因小鼠比正常小鼠Tie2基因的表达高约2. 5倍。Tie2蛋白在转基因小鼠牙周组织中显著高表达,差异具有统计学意义(P<0. 05)。GFP荧光提示Tie2在转基因小鼠牙周组织血管周围表达相对较高。转基因小鼠和正常小鼠牙周组织中GNPs的累积量随粒径增大而减少,正常小鼠牙周组织中GNPs的累积量明显高于转基因小鼠,差异具有统计学意义(P<0. 05)。结论 Tie2基因高表达不利于GNPs扩散。Objective To investigate the effect of Tie2 gene expression on diffusion of gold nanoparticles(GNPs) into periodontal tissues in mice. Methods The expression of Tie2 mRNA and Tie2 protein in periodontal tissues of normal and transgenic BALB/c mice was detected by real-time RT-PCR and Western Blot.The expression of GFP in periodontal tissue of transgenic Tie2-GFP mice was detected by fluoromicroscopy with frozen section.Mice were injected with GNPs(1 g/kg) with diameters of 20,40,60 and 80 nm,respectively;and the content of gold(Au) in periodontal tissue was detected by ICP-AES. Results The mRNA expression in periodontal tissue of Tie2 transgenic mice was 2.5 times higher than that in normal mice;and the expression of Tie2 protein was also higher in the periodontal tissues of transgenic mice( P <0.05).GFP fluorescence indicated that Tie2 protein was higher expressed around the vessels in periodontal tissue of transgenic mice.The contents of GNPs in the periodontal tissue of transgenic mice and normal mice were decreased with the increase of particle size.The amount of GNPs in periodontal tissue of normal mice was significantly higher than that of transgenic mice( P <0.05). Conclusion High expression of Tie2 gene is associated with the decreased diffusion of gold nanoparticles into periodontal tissue.

关 键 词：Tie2基因 转基因小鼠 纳米金颗粒 牙周组织 

分 类 号：R781[医药卫生—口腔医学]