T淋巴细胞亚群、VEGF、MMP-14检测对肝癌射频消融术后残癌临床评估的价值  

Clinical evaluation of T lymphocyte subsets and VEGF, MMP-14 in residual carcinoma after radiofrequency ablation of liver cancer

作　　者：霍传红[1] 张淑红[1] Huo Chuanhong;Zhang Shuhong(Dezhou People's Hospital, Dezhou 253014, China)

机构地区：[1]德州市人民医院,253014

出　　处：《国际医药卫生导报》2019年第8期1186-1189,共4页International Medicine and Health Guidance News

基　　金：山东省医药卫生科技发展计划项目(2017WS541).

摘　　要：目的 检测原发性肝癌(HCC)患者采用射频消融术(RFA)后,组织内T淋巴细胞亚群、血管内皮生长因子(VEGF)、金属蛋白酶-14(MMP-14)水平,分析以上指标对残癌的临床评估价值.方法 选取2017年11月至2018年10月间在本院行RFA手术的HCC患者60例作为观察组,均经肝穿刺病理活检确诊,收集观察组RFA术后32例非残癌组织(观察A组)及28例残癌组织(观察B组);收集同期在本院行单纯手术切除的40例HCC组织标本作为对照组.采用流式细胞仪检测两组患者外周血CD3+T细胞、CD4+T细胞、CD4+/CD8+细胞;采用免疫组化法检测VEGF及MMP-14阳性表达.结果 治疗后,对照组和观察组外周血CD4+及CD4+/CD8+水平均显著低于治疗前,而CD8+则显著高于治疗前(P<0.01);治疗后1个月,观察A组CD3+水平为(73.7±17.1)%、CD4+为(41.2±10.5)%、CD8+为(21.3±5.6)%、CD4+/CD8+为(2.0±0.6),均明显优于对照组及观察B组,组间差异均有统计学意义(P<0.05).治疗后,观察B组VEGF蛋白阳性人数为24例(85.7%)、MMP-14蛋白阳性人数为22例(78.6%),均显著高于对照组及观察A组,组间差异均有统计学意义(P<0.05).结论 RFA术后,HCC无残癌组织的T淋巴细胞亚群有明显升高,而残癌组织中VEGF及MMP-14蛋白水平较高,临床可以通过检测T淋巴细胞亚群、VEGF及MMP-14水平判断HCC是否存在残留病灶.Objective To detect the levels of T lymphocyte subsets,vascular endothelial growth factor (VEGF),metalloproteinase-14 (MMP-14) in patients with primary liver cancer (HCC) after radiofrequency ablation (RFA),to evaluate the clinical evaluation value of the above indexes in residual carcinoma.Methods From November 2017 to October 2018,60 HCC patients undergoing RFA operation in our hospital were selected as the observation group.All the patients were diagnosed by liver biopsy.32 cases of non-residual carcinoma tissues (group A) and 28 cases of residual carcinoma tissues (group B) were collected after RFA operation in the observation group.A total of 40 HCC tissue specimens were collected as control group after simple surgical resection in our hospital during the same time period.CD3+ T cells,CD4+ T cells,CD4+/CD8+ in the peripheral blood were detected by flow cytometry,and the positive expression of VEGF and MMP-14 were detected by immunohistochemical method.Results After treatment,the levels of CD4+ and CD4+/CD8+ in the control group and the observation group were significantly lower than those before treatment,while CD8+ was significantly higher than that before treatment (P<0.01).One month after treatment,the levels of CD3+,CD4+,CD8+,and CD4+/CD8+ in group A were (73.7± 17.1)%,(41.2± 10.5)%,(21.3±5.6)%,and (2.0±0.6),respectively,which were significantly better than those in the control group and group B,with statistically significant differences among the three groups (P<0.05).After treatment,24 cases (85.7%) were positive for VEGF protein and 22 cases (78.6%)were positive for MMP-14 protein in group B,which were significantly higher than those in group A and the control group,with statistically significant differences (P<0.05).Conclusion After RFA,the T lymphocyte subsets of HCC without residual carcinoma were significantly higher,while the levels of VEGF and MMP-14 protein were higher in residual carcinoma tissues.It may be possible to determine the presence of residual lesions in HCC by detecting T lympho

关 键 词：淋巴细胞亚群 VEGF 射频消融术 MMP-14 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]