结直肠癌术后患者化疗前后外周血NKT及NK细胞表达分析  

Expression of peripheral blood NKT and NK cells in postoperative colorectal cancer patients before and after chemotherapy

作　　者：史钟[1] 郁肖夫[1] 朱静[1] 袁梅琴[1] 吕汪霞[1] 冯婷婷[1] 钟海均[1] SHI Zhong;YU Xiaofu;ZHU Jing;YUAN Meiqin;LV Wangxia;FENG Tingting(Department of Abdominal Oncology,Zhejiang Cancer Hospital,Hangzhou 310022,China)

机构地区：[1]浙江省肿瘤医院腹部肿瘤内科,浙江杭州310022

出　　处：《中国现代医生》2019年第10期86-89,共4页China Modern Doctor

基　　金：国家自然科学基金青年基金(81602583);浙江省医药卫生科技项目(2016KYB043)

摘　　要：目的观察结直肠癌术后患者化疗前后外周血淋巴细胞亚群的变化,评估患者免疫功能状态变化。方法纳入浙江省肿瘤医院2016年1～12月住院的结直肠癌根治术后Ⅱ～Ⅲ期患者74例,健康对照组为25例同期我院就诊的非恶性肿瘤患者,采用流式细胞术分析辅助化疗第1、6、8周期前1 d外周血淋巴细胞亚群比例。结果结直肠癌根治术后患者外周血中CD3+(P=0.1532)、CD3+CD4+(P=0.1107)、CD3+CD8+(P=0.2576)T细胞与健康对照组比较无差异(P>0.05),而CD3+CD56+NKT细胞(P=0.0210)及CD3-CD56+NK细胞(P=0.0045)较健康对照者显著升高(P<0.05)。术后辅助化疗6周期前NK、NKT细胞比例均无显著变化,P>0.05。8周期前NKT细胞比例无显著变化(P>0.05),NK细胞比例仅轻度下降(19.10±4.63 vs 20.31±4.66,P=0.046)。结论结直肠癌根治术后患者NK及NKT细胞比例较健康人显著升高,且辅助化疗后表达率保持稳定。Objective To observe the changes of peripheral blood lymphocyte subsets in patients with postoperative colorectal cancer before and after chemotherapy,and to evaluate the changes of immune function status.Methods 74 patients with stageⅡ-Ⅲcolorectal cancer after radical resection admitted in Zhejiang Cancer Hospital from January to December 2016 were enrolled.25 non-malignant patients in the same period in our hospital were selected as the control group.Flow cytometry was used to analyze the proportion of peripheral blood lymphocyte subsets at one day before the first,sixth and eighth cycles of adjuvant chemotherapy.Results There were no differences in CD3^+(P=0.1532),CD3^+CD4^+(P=0.1107),CD3^+CD8^+(P=0.2576)T cells in the peripheral blood between patients with colorectal cancer after radical operation and control group(P>0.05).While CD3^+CD56^+NKT cells(P=0.0210)and CD3^-CD56^+NK cells(P=0.0045)in the patients with colorectal cancer after radical operation were significantly higher than those of the control group(P<0.05).There was no significant change in the proportion of NK and NKT cells before 6 cycles of postoperative adjuvant chemotherapy(P>0.05).There was no significant change in the proportion of NKT cells before 8 cycles(P>0.05),and the proportion of NK cells only decreased slightly(19.10±4.63 vs 20.31±4.66,P=0.046).Conclusion The proportion of NK and NKT cells in patients with colorectal cancer after radical resection was significantly higher than that in healthy people,and the expression rate remains stable after adjuvant chemotherapy.

关 键 词：结直肠癌 淋巴细胞亚群 NK细胞 NKT细胞 

分 类 号：R730.4[医药卫生—肿瘤]