下调微小RNA-146b表达对甲状腺癌细胞增殖与凋亡的影响  

The role of microRNA-146b on proliferation and apoptosis of thyroid carcinoma

作　　者：曲鑫[1] 邸旭[1] 王佳鑫 张海超[1] 李彩霞[1] Qu Xin;Di Xu;Wang Jiaxin;Zhang Haichao;Li Caixia(Department of Mammary and Thyroid Surgery, Tianjin Fourth Central Hospital, Tianjin 300143, China;Department of Head and Neck Cancer Surgery, Beijing Cancer Hospital, Beijing 100142, China)

机构地区：[1]天津市第四中心医院乳腺甲状腺外科,300143 [2]北京肿瘤医院头颈部肿瘤外科,100142

出　　处：《中华实验外科杂志》2019年第4期626-628,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨下调微小RNA(miRNA,miR)-146b表达对甲状腺癌细胞增殖与凋亡的影响。方法应用Lipofectamine^TM 3000将miR-146b inhibitor、miR-146b NC转染到甲状腺癌细胞SW579,并测定miR-146b表达量,细胞活力,细胞凋亡,细胞周期及B细胞淋巴瘤/白血病-2(bcl-2)、bcl-2相关X蛋白(bax),细胞周期素D1(Cyclin D1)、细胞周期蛋白依赖性激酶4(CDK4)蛋白表达量及核转录因子-κB(NF-κB) p65磷酸化水平。结果miR-146b inhibitor组(0.38±0.05)miR-146b表达量低于miR-146b NC组(1.00±0.09)(P<0.05)。miR-146b inhibitor组(0.36±0.04)细胞活力低于miR-146b NC组(0.59±0.06)(P<0.05)。miR-146b inhibitor组细胞凋亡率(34.58±3.50)%高于miR-146b NC组(4.28±0.43)%(P<0.05),miR-146b inhibitor组细胞周期G1期(44.32±4.43)%长于miR-146b NC组(34.08±3.42)%(P<0.05)。miR-146b inhibitor组中bcl-2、Cyclin D1、CDK4蛋白表达量及NF-κB p65磷酸化水平低于miR-146b NC组(P<0.05),miR-146b inhibitor组中bax表达量高于miR-146b NC组(P<0.05)。结论下调miR-146b可能通过阻断NF-κB信号通路抑制SW579甲状腺癌细胞的增殖,并诱导细胞凋亡。Objective To explore the role of microRNA (miRNA, miR)-146b on proliferation and apoptosis of thyroid carcinoma. Methods After miR-146b inhibitor and miR-146b NC were transfected into SW579 cell by liposome LipofectamineTM3000, the expression of miR-146b, cell viability, cell apoptotic rate, cell cycle, the expression of B cell lymphoma/lewkmia-2 (bcl-2), bcl-2 related X protein (bax), cyclin dependent kinases (CDK4), phosphorylation of nuclear factor kappa B (NF-κB) p65 was detected. Results The expression of miR-146b in miR-146b inhibitor group (0.38±0.05) was lower than that in miR-146b NC (1.00±0.09)(P<0.05). Cell viability in miR-146b inhibitor group (0.36±0.04) was lower than that in miR-146b NC group (0.59±0.06)(P<0.05). Cell apoptosis rate (34.58±3.50)% was higher than that in miR-146b NC group (4.28±0.43)%(P<0.05). The cell cycle in miR-146b inhibitor group (44.32±4.43)% was longer than miR-146b NC group (34.08±3.42)%(P<0.05). The expression of bcl-2, Cyclin D1 and CDK4, phosphorylation of NF-κB p65 in miR-146b inhibitor group was lower than that in miR-146b NC group (P<0.05), the expression of bax in miR-146b inhibitor group was higher than that in miR-146b NC group (P<0.05). Conclusion MiR-146b inhibitor might inhibit the proliferation of SW579 cell and induce cell apoptosis via blocking NF-κB signaling pathway.

关 键 词：微小RNA-146b 甲状腺癌 增殖 凋亡 

分 类 号：R736.1[医药卫生—肿瘤]