植物功能盐对小鼠急性化学性肝损伤的防护实验研究  

Experimental Study on Protective Effects of Plant Functional Salts on Chemical Liver Injury in Mice

作　　者：陈亮 周沫希 严雅丽 张英[1] CHEN Liang;ZHOU Mo-xi;YAN Ya-li;ZHANG Ying(Key Laboratory for Agro-products Processing Technology Research in Zhejiang Province, ZhejiangR & D Center for Food Processing Technology and Equipment, College of BiosystemsEngineering and Food Science, Zhejiang University, Hangzhou 310058, China)

机构地区：[1]浙江大学生物系统工程与食品科学学院浙江省农产品加工技术研究重点实验室浙江省食品加工技术与装备工程研究中心,杭州310058

出　　处：《中国调味品》2019年第5期28-32,共5页China Condiment

摘　　要：目的:采用四氯化碳(CCl_4)诱导的急性化学性肝损伤动物模型,评价3种植物功能盐(竹盐、松盐和梅盐)对试验小鼠急性化学性肝损伤的防护作用。方法:将45只5周龄的C57BL/6健康雄性小鼠适应性喂养7天后随机分成9组,末次给药1h后,模型组和试验组小鼠均腹腔注射0.1%的CCl_4橄榄油溶液,剂量为10mL/kg·bw,正常组给予等体积的橄榄油作为对照。禁食过夜,16h后取血液和肝脏样本,测定血清中天冬氨酸转氨酶(AST)的活性和谷氨酸转氨酶(ALT)的活性;测定肝组织匀浆中丙二醛(MDA)含量以及谷胱甘肽(GSH)、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力;同时,观察试验小鼠肝组织的病理损伤程度。结果:竹盐、松盐和梅盐均可不同程度地降低血清转氨酶(AST和ALT)的水平,降低肝脏的MDA含量、提高GSH水平及T-SOD活力,并减轻肝脏的病理性损伤。结论:以竹盐为代表的3种植物功能盐对CCl_4诱导的小鼠急性化学性肝损伤均有一定的保护作用。Objective:The protective effects of three plant functional salts (bamboo salt, pine salt and plum salt) on acute chemical liver injury in mice induced by carbon tetrachloride (CCl 4 ) are studied. Methods: Forty- five healthy C57BL/6 male mice are randomly divided into 9 groups after 7 days' adaptive feeding. 1 h after the last dosing, the mice in the model group and the experimental group are intraperitoneally injected with 0.1% CCl 4 olive oil solution at a dose of 10 mL/kg·bw, and the normal group with the same volume of olive oil as the control group. All mice are forbidden to eat overnight, and blood and liver samples are taken after 16 h. The activities of alanine transaminase(ALT) and aspartate aminotransferase (AST), the content of malondialdehyde (MDA) and glutathione (GSH) in liver homogenate, and the activities of total superoxide dismutase(T-SOD) and GSH-px in liver homogenate are measured. At the same time, the pathological damage degree of liver tissues in mice are examined.Results:Bamboo salt, pine salt and plum salt could reduce the activities of serum transaminase (AST and ALT), reduce the content of MDA in liver, increase GSH level and SOD activity, and alleviate the pathological damage of liver.Conclusion:Three kinds of plant functional salts all show certain functions on chemical liver injury in mice induced by CCl 4.

关 键 词：植物功能盐 护肝功效 四氯化碳 动物试验 急性化学性肝损伤 

分 类 号：TS207.3[轻工技术与工程—食品科学]