AaGroEL对OMECs增殖的影响及其作用机制研究  

Effect of AaGroEL on proliferation of OMECs and its mechanism

作　　者：雷先会 赵蔚萍[1] 杨森[1] Lei Xianhui;Zhao Weiping;Yang Sen(Department of Stomatology, Suining Central Hospital, Suining, Sichuan, 629000, China)

机构地区：[1]遂宁市中心医院口腔科,四川遂宁629000

出　　处：《西南国防医药》2019年第5期526-528,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：四川省科技计划项目(2018FZ0113)

摘　　要：目的探讨HSP60的细菌同源物GroEL(AaGroEL)对口腔黏膜上皮细胞(OMECs)增殖的影响及其作用机制。方法从15名正常牙周健康者的口腔黏膜中分离OMECs,随机分为HSP60组和对照组;从放线共生放线杆菌(A.a)中提取并纯化AaGroEL,将0.25 g/ml的AaGroEL加入HSP60组,对照组则加入溶媒。分别于孵育0、6、12、24、48、72、96、144 h时,采用细胞增殖试剂盒检测各组细胞增殖抑制率;于孵育12 h和96 h时,采用实时定量PCR检测细胞内信号调节激酶(ERK)、丝裂原激活蛋白激酶p38 (p38)、蛋白磷酸酶1(PP1)和热休克蛋白60(HSP60)的表达水平。结果与对照组相比,AaGroEL与OMECs孵育12 h前,显著促进OMECs的增殖;而孵育12 h后,则显著抑制OMECs的增殖。与对照组对比,AaGroEL处理12 h时,可显著增加ERK1和ERK2的表达,抑制p38和PP1的表达(P <0.05);而处理96 h时,ERK1和ERK2的表达降低,p38和PP1的表达均增加(P <0.05)。AaGroEL处理12 h时,可抑制热刺激诱导的胞内HSP60表达的增加(P <0.05)。结论 AaGroEL短期处理促进OMECs增殖,而长期处理则抑制OMECs增殖,其机制可能与AaGroEL双调控MAPK信号通路有关。Objective To explore the effect of GroEL(AaGroEL), a bacterial homologue of HSP60, on the proliferation of oral mucosal epithelial cells(OMECs) and its mechanism. Methods OMECs were isolated from oral mucosa of 15 healthy periodontal patients and randomly divided into a HSP60 group and a control group. AaGroEL was extracted and purified from Actinobacillus actinomycetemcomitans(Aa). 0.25 g/ml of AaGroE was added to the HSP60 group, while the control group was added with the solvent. At 0, 6, 12, 24, 48, 72, 96 and 144 hours of incubation, the inhibition rate of cell proliferation was detected by cell proliferation kit. At 12 and 96 hours of incubation, the expression levels of signal-regulated kinase(ERK), mitogen-activated protein kinase p38(p38), protein phosphatase 1(PP1) and heat shock protein 60(HSP60) were detected by real-time quantitative PCR.Results Compared with the control group, AaGroEL significantly promoted the proliferation of OMECs 12 hours before the incubation, while AaGroEL significantly inhibited the proliferation of OMECs 12 hours after the incubation. Compared with the control group, AaGroEL significantly increased the expression of ERK1 and ERK2, inhibited the expression of p38 and PP1(P < 0.05) at12 h, while at 96 h, the expression of ERK1 and ERK2 decreased and the expression of p38 and PP1 increased(P < 0.05). At 12 hours, AaGroEL inhibited the increase of HSP60 expression induced by heat stimulation(P < 0.05). Conclusion Short-term treatment of AaGroEL can promote the proliferation of OMECs, while long-term treatment will inhibit the proliferation of OMECs. The mechanism may be related to the dual regulation of MAPK signaling pathway by AaGroEL.

关 键 词：热休克蛋白60 GROEL 放线共生放线杆菌 口腔黏膜 上皮细胞 

分 类 号：R781.5[医药卫生—口腔医学]