老年脑杏仁核嗜银颗粒病理观察  

Pathological features of argyrophilic grains in amygdaloid nuclei of aging brain

作　　者：王圆圆 朱明伟[1] 王鲁宁[1] 张红红[2] 胡亚卓[2] 韩志涛[2] 张莹晗 Wang Yuanyuan;Zhu Mingwei;Wang Luning;Zhang Honghong;Hu Yazhuo;Han Zhitao;Zhang Yinghan(NationalClinical Research Center for Geriatric Diseases,Beijing 100853,China)

机构地区：[1]解放军总医院第二医学中心神经内科国家老年疾病临床医学研究中心,北京100853 [2]解放军总医院第二医学中心老年医学研究所,北京100853 [3]新乡医学院神经精神医学研究院

出　　处：《中华老年心脑血管病杂志》2019年第5期499-501,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：全军保健专项科研课题(15BJZ38)

摘　　要：目的认识正常脑老化及阿尔茨海默病、帕金森病和进行性核上性麻痹杏仁核嗜银颗粒病理特征。方法收集14例解放军总医院临床病理资料完整的老年尸体解剖病例杏仁核标本,其中5例阿尔茨海默病,3例帕金森病,2例进行性核上性麻痹和4例正常老年脑。行苏木精-伊红染色、劳克坚牢蓝髓鞘染色及Gallyas-Braak银染色检查,相应切片行β淀粉样蛋白抗体、tau抗体、α突触核蛋白抗体和P62抗体免疫组织化学染色,观察嗜银颗粒病理特征。结果在Gallyas-Braak银染色,14例杏仁核组织中,发现4例存在嗜银颗粒结构,阳性率为28.6%,其中2例阿尔茨海默病,1例进行性核上性麻痹和1例正常老年脑。其蛋白质病理染色均显示为tau及P62阳性。结论杏仁核嗜银颗粒病变在正常老年脑、阿尔茨海默病、进行性核上性麻痹等老化相关神经变性疾病并非少见,可能对阿尔茨海默病及进行性核上性麻痹等疾病临床认知功能障碍症状产生叠加效应。Objective To study the pathological features of argyrophilic grains in normal aging brain,AD,PD and progressive superior nuclear paralysis patients.Methods Brain tissue samples taken from 5 AD,3 PD,2 progressive superior nuclear paralysis patients with complete clinicopathological data and 4 normal aging brain subjects were stained with HE,Luxol fast blue and Gallyas-Braak silver respectively.Aβ,tau,α-synuclein and P62 antibodies were detected by microscopy with immunohistochemical staining.The pathological features of argyrophilic grains were recorded.Results The Gallyas-Braak silver staining showed argyrophilic grain structure in 4 out of the 14 amygdaloid nucleus tissue samples(2 from AD patients,1 from progressive superior nuclear paralysis patients and 1 from normal aging brain patients)with a positive rate of 28.6%. The immunohistochemical staining showed positive tau and P62 antibodies.Conclusion Argyrophilic grain lesion is not uncommon in aging-related neurodegenerative diseases such as normal aging brain,AD and progressive superior nuclear paralysis and can thus produce its superposition effect on the clinical symptoms of cognitive impairment in AD and progressive superior nuclear paralysis patients.

关 键 词：阿尔茨海默病 帕金森病 核上麻痹 进行性 杏仁核 认知障碍 淀粉样Β蛋白 

分 类 号：R742[医药卫生—神经病学与精神病学]