高通量测序分析北仑河口红树林潮间带底栖真核生物分布  

Distribution of benthic eukaryote communities in intertidal zone of mangrove in the Beilun Estuary by high-throughput sequencing analysis

作　　者：吴鹏[1] 时小军[1] 吕向立[1] 高阳[1] 李海涛[1] 张敬怀[1] WU Peng;SHI Xiao-jun;LV Xiang-li;GAO Yang;LI Hai-tao;ZHANG Jing-huai(South China Sea Environmental Monitoring Center, SOA, Guangzhou 510301,China)

机构地区：[1]国家海洋局南海环境监测中心,广东广州510301

出　　处：《应用海洋学学报》2019年第2期232-238,共7页Journal of Applied Oceanography

基　　金：国家自然科学基金资助项目(41776172;41606191;41606136);广东省自然科学基金资助项目(2014A030310495)

摘　　要：为了解红树林不同潮位沉积物中底栖真核生物群落分布,基于18S rRNA基因采用高通量测序方法分析了广西北仑河口陆缘、林中和海缘3个潮位红树林沉积物中底栖生物群落结构。结果表明,北仑河口潮间带红树林沉积物中底栖生物多样性丰富,Shannon-Wiener指数变化范围在6. 08～6. 73之间; PCA分析表明潮间带中底栖生物群落差异较大,陆缘红树林中扁形动物、节肢动物和软体动物相对丰度较高,林中区域中纤毛虫、环节动物和轮虫相对丰度较高,海缘红树林中硅藻相对丰度较高;红树林中主要OTUs有桡足类的太平洋纺锤水蚤(Acartia pacifica)、硅藻类的海链藻(Thalassiosira sp.)、纤毛虫类的前管虫(Prorodon teres)、多毛类的小头虫(Capitella sp.)。高通量测序方法能较全面反映红树林区微型/小型底栖生物群落,研究结果为丰富红树林底栖生物群落研究和解析底栖生物在红树林生态系统发挥的作用提供基础数据。To reveal the distribution of benthic eukaryote communities at three sites in intertidal zone of mangrove in Beilun Estuary, China, we investigated the community structure of benthic eukaryotes by Illumina high throughput DNA pyrosequencing of the 18S rRNA gene. The results showed that benthic eukaryote communities in mangrove sediments of Beilun Estuary were highly diverse. Shannon-wiener index of the different intertidal zones ranged from 6.08 to 6.73. The PCA result demonstrated that the species compositions were significantly different among the intertidal zone communities. The relative abundances of Platyhelminthes, Arthropoda and Mollusca were higher in the upper zone mangrove. However, some groups such as Ciliophora, Annelida and Rotifera were more abundant in the middle zone mangrove, whereas Diatomea was found more in the lower part mangrove. Of the total OTUs observed for benthic eukaryotes, the dominant OTUs were similar to 18S rRNA gene sequences reported from Acartia pacifica , Thalassiosira sp., Prorodon teres and Capitella sp. The results showed that the method of DNA high-throughput sequencing can comprehensively estimate the benthic eukaryote communities in mangrove sediments and shed insights into the function of benthic eukaryote communities of the mangrove ecosystem.

关 键 词：海洋生物学 红树林 潮间带 底栖真核生物 群落结构 高通量测序 北仑河口 

分 类 号：P735[天文地球—海洋生物学]