血清表面增强拉曼光谱应用于口腔鳞状细胞癌患者临床分期的研究  

Research on the surface-enhanced Raman spectrum of blood serum for clinical staging of oral squamous cell carcinoma

作　　者：闫冰[1,2,3] 骆献阳 谭迎赟[4] 关丽梅 薛丽丽 Yan Bing;Luo Xianyang;Tan Yingyun;Guan Limei;Xue Lili(Dept. of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China;School of Medicine, Xiamen University, Xiamen 361003, China;Xiamen Key Laboratory of Otolaryngology Head and Neck Surgery, Xiamen 361003, China;Dept. of Implant, Stomatological Hospital of Chongqing Medical University, Chongqing 401120, China;Dept. of Stomatology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China)

机构地区：[1]厦门大学附属第一医院耳鼻咽喉头颈外科,厦门361003 [2]厦门大学医学院,厦门361003 [3]厦门市耳鼻咽喉头颈外科重点实验室,厦门361003 [4]重庆医科大学口腔医院种植科,重庆401120 [5]厦门大学附属第一医院口腔科,厦门361003

出　　处：《国际口腔医学杂志》2019年第3期283-287,共5页International Journal of Stomatology

基　　金：国家自然科学基金(81502584);福建省医学创新课题(2015-CXB-35);福建省自然科学基金(2017J01357);厦门市科技计划项目(3502Z20174062)~~

摘　　要：目的应用表面增强拉曼光谱技术研究口腔鳞状细胞癌患者血清,并建立临床分期鉴别诊断模型。方法收集144例口腔鳞状细胞癌患者血清,按照早期组、中期组及晚期组进行分组检测血清表面增强拉曼光谱,比较光谱差异,应用主成分分析法及线性判别函数法建立鉴别诊断模型,对不同分组光谱数据进行鉴别诊断。结果共获得血清拉曼光谱144例,其中早期组59例,中期组40例及晚期组45例。血清光谱差异主要表现在721～727、931～932、1129～1133、1264～1266、1367～1371 cm^(-1)处谱峰强度差异,主要与血清中脂类、蛋白质及核酸成分和结构有关。鉴别诊断模型总体准确率达80%以上,经过交互验证后准确率为70%以上。结论表面增强拉曼光谱检测口腔鳞状细胞癌患者血清为口腔鳞状细胞癌临床分期及预后判断提供了一种简便、准确的新方法。Objective To evaluate the surface-enhanced Raman spectrum of the blood serum of oral squamous cell carcinoma patients and establish a diagnostic model for clinical staging. Methods Serum samples were obtained from a total of 144 patients with oral squamous cell carcinoma and classified into three groups, namely, the early cancer group, the mid-term cancer group, and the advanced cancer group. Principal component and linear discriminant analyses were utilized to establish a diagnostic model with which to classify the different groups based on spectral differences. Results A total of 144 spectra were generated from the samples. The main differences found involved peaks at 721-727, 931-932, 1 129-1 133,1 264-1 266, and 1 367-1 371 cm^-1, which can be assigned to proteins, lipids, and nucleic acids. The total accuracy of the established model exceeded 80%, and its cross-validation accuracy exceeded 70%. Conclusion Surface-enhanced Raman spectroscopy could be applied to detect the clinical stages of oral squamous cell carcinoma based on blood serum.

关 键 词：口腔鳞状细胞癌 表面增强拉曼光谱 临床分期 诊断 

分 类 号：R782[医药卫生—口腔医学]