慢病毒介导的RNA干扰沉默PIM2表达对结肠癌HT29细胞增殖、侵袭、迁移和凋亡的影响  

Effect of lentivirus-mediatedRNA interference silencing PIM2 expression on proliferation, invasion, migration and apoptosis of human colorectal cancer HT29 cells

作　　者：王磊[1] 时军利[1] 王春青[1] 李炳庆[1] WANG Lei;SHI Junli;WANG Chunqing;LI Bingqing(Department of Gastroenterology, the Affiliated Hospital of Chengde Medical College, Chengde 067000, China)

机构地区：[1]承德医学院附属医院消化内科,河北承德067000

出　　处：《胃肠病学和肝病学杂志》2019年第5期528-531,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：河北省医学科学研究重点课题计划(20150021)

摘　　要：目的探讨沉默PIM2表达对结肠癌HT29细胞增殖、侵袭、迁移和凋亡的影响。方法采用实时荧光定量PCR和Western blotting方法检测人正常结肠上皮NCM460细胞和结肠癌HT29细胞中PIM2 mRNA和蛋白表达。以含PIM2 shRNA慢病毒载体感染HT29细胞,采用实时荧光定量PCR和Western blotting方法检测感染效果,CCK-8法、Transwell实验和流式细胞仪分别检测细胞的增殖、侵袭、迁移和凋亡能力。结果与NCM460细胞相比,HT29细胞中PIM2 mRNA和蛋白表达明显升高(P<0.05)。PIM2 shRNA慢病毒感染使HT29细胞中PIM2 mRNA和蛋白表达显著降低,显著抑制SGC7901细胞的增殖、侵袭和迁移并促进细胞凋亡(P<0.05)。结论 PIM2在结肠癌HT29细胞中高表达,干扰其表达可抑制HT29细胞增殖、侵袭和迁移,并促进细胞凋亡。Objective To investigate the effect of silencing PIM2 expression on proliferation, invasion, migration and apoptosis of colorectal cancer HT29 cells. Methods The expressions of PIM2 mRNA and protein in human normal colon epithelial NCM460 cells and colorectal cancer HT29 cells were detected by Real-time fluorescent quantitative PCR and Western blotting. HT29 cells were infected with lentiviral vector containing PIM2 shRNA, and the infection effect was tested by Real-time fluorescent quantitative PCR and Western blotting. The proliferation, invasion, migration and apoptosis of cells were measured by CCK-8 method, Transwell test and flow cytometry. Results Compared with NCM460 cells, the expression of PIM2 in HT29 cells was significantly increased ( P <0.05). The expression of PIM2 in HT29 cells was reduced significantly, and the proliferation, invasion and migration of SGC7901 cells were inhibited, and cell apoptosis was promoted remarkably by PIM2 shRNA lentivirus infection. Conclusion PIM2 is highly expressed in HT29 cells of colorectal cancer. Interference of PIM2 expression can inhibit the proliferation, invasion and migration of HT29 cells and promote cell apoptosis.

关 键 词：结肠癌 PIM2 增殖 侵袭 迁移 凋亡 

分 类 号：R735.3[医药卫生—肿瘤]