甘美达凝胶对大鼠口腔溃疡愈合及溃疡组织中TNF-α、IL-6、EGF表达的影响  

Effect of Kamistad gel on oral ulcer healing and TNF-α, IL-6 and EGF expression in ulcer tissue of rats

作　　者：王艺衡 吴迎涛 于习习 李璐璐[3] 邓松松 刘梦佳 王万春 WANG Yiheng;WU Yingtao;YU Xixi;LI Lulu;DENG Songsong;LIU Mengjia;WANG Wanchun(Stomatology College of Weifang Medical University, Weifang 261053, China;Department of Oral Medicine, Qingdao Stomatological Hospital, Qingdao 266001, China;Stomatology College of Qingdao University, Qingdao 266071, China)

机构地区：[1]潍坊医学院口腔医学院,山东潍坊261053 [2]青岛市口腔医院黏膜科,山东青岛266001 [3]青岛大学口腔医学院,山东青岛266071

出　　处：《口腔疾病防治》2019年第5期293-299,共7页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：山东省中医药科技发展计划项目(2017344)

摘　　要：目的探讨甘美达凝胶对大鼠口腔溃疡愈合及溃疡组织肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)和表皮生长因子(epidermal growth factor,EGF)表达的影响,为其临床应用提供实验依据。方法采用化学烧灼法建立大鼠口腔溃疡动物模型,随机分为甘美达组(甘美达凝胶)、贝复新组(贝复新凝胶)、利多卡因组(利多卡因乳膏)、空白对照组(生理盐水),每组12只。各组在溃疡治疗前及治疗后1、3、5 d测量各组大鼠溃疡面积;治疗后1 d,口内滴注辣椒素记录大鼠3 min内拭嘴行为时间评估其疼痛程度;治疗后5 d,切取大鼠溃疡组织,HE染色观察组织病理学变化,免疫组化法和酶联免疫吸附法检测溃疡组织中TNF-α、IL-6和EGF的表达。结果治疗后1 d,甘美达组的辣椒素所致大鼠拭嘴行为时间明显小于空白对照组及贝复新组(P <0.05),与利多卡因组差异无统计学意义(P> 0.05)。治疗后5 d,甘美达组的溃疡面积明显小于空白对照组及利多卡因组(P <0.05),与贝复新组差异无统计学意义(P> 0.05)。治疗后5d,大鼠口腔溃疡HE染色切片显示,甘美达组较利多卡因组和空白对照组炎症细胞明显减少,成纤维细胞明显增生,上皮增生情况良好,与贝复新组无差异。治疗后5 d,各组大鼠溃疡组织中TNF-α、IL-6、EGF的表达水平比较,差异均有统计学意义(P <0.05)。相较于空白对照组和利多卡因组,甘美达组TNF-α降低,EGF的表达上调,差异具有统计学意义(P <0.05);甘美达组与贝复新组以上3种因子的表达差异无统计学意义(P> 0.05)。结论甘美达凝胶对实验性口腔溃疡具有较好的抗炎、止痛、促进愈合的作用。Objective To investigate the effect of Kamistad gel on oral ulcer healing and the expression of tumor necrosis factorα(TNF-α),interleukin 6(IL-6)and epidermal growth factor(EGF)after oral administration in ulcer tissue of rats and to provide animal experimental data for the clinical application of Kamistad gel.Methods The oral ulcer rat model was established by chemical cauterization.The rats were randomly divided into four groups:Kamistad group(Kamistad gel),befuxin group(befuxin gel),lidocaine group(lidocaine cream),blank control group(normal saline),with 12 rats in each group.The ulcer area of the rats in each group was measured before and 1,3 and 5 days after treatment;at 1 day after treatment,the duration of swabbing behavior within 3 minutes of intraoral capsaicin infusion was recorded to evaluate the degree of pain;the ulcer tissue was collected at 5 days after treatment,and the histopathological changes were observed by HE staining,the expression of TNF-α,IL-6 and EGF in the ulcer tissue was detected by immunohistochemistry and ELISA.Results At 1 day after treatment,the duration of mouth wiping induced by capsaicin was significantly shorter in the Kamistad group than in the blank control and befuxin groups(P<0.05),but there was no significant difference between the Kamistad and lidocaine groups(P>0.05).At 5 days after treatment,the ulcer area was significantly smaller in the Kamistad group than in the blank control and lidocaine groups(P<0.05),and there was no significant difference between the Kamistad and befuxin groups(P>0.05).At 5 days after treatment,H&E staining of the oral ulcer tissue sections showed significantly reduced levels of inflammatory cells and significantly proliferated fibroblasts and better epithelial hyperplasia in the Kamistad group compared with those in the lidocaine and blank control groups,and there were no differences between the Kamistad and befuxin groups.At 5 days after treatment,the expression levels of TNF-α,IL-6 and EGF in the ulcer tissue of rats in each group were s

关 键 词：口腔溃疡 甘美达凝胶 大鼠 肿瘤坏死因子-α 白细胞介素-6 表皮生长因子 

分 类 号：R78[医药卫生—口腔医学]