一种小型化低成本免疫荧光分析仪的设计  

Design of a Miniaturized and Low-Cost Immunofluorescence Analyzer

作　　者：沈增贵[1] 邓红玉 林学祥 SHEN Zenggui;DENG Hongyu;LIN Xuexiang(Department of Equipment, Nanfang Hospital, Southern Medical University, Guangzhou Guangdong 510515, China;Departmentof Rehabilitation, The Third Affilliated Hospital of Southern Medical University, Guangzhou Guangdong 510000, China;Instituteof Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, ShenzhenGuangdong 518055, China)

机构地区：[1]南方医科大学南方医院设备器材科,广东广州510515 [2]南方医科大学第三附属医院康复科,广东广州510000 [3]中国科学院深圳先进技术研究院生物医学与健康工程研究所,广东深圳518055

出　　处：《中国医疗设备》2019年第5期54-57,64,共5页China Medical Devices

基　　金：广州市科技计划(No.201604016041)

摘　　要：目的研制一款小型化、低成本、带自动抛样功能的免疫荧光分析仪。方法该免疫荧光分析仪采用非共聚焦方式的光路设计。光源位于光模块两侧,光电二极管位于试纸条正上方。光模块使用微动开关作为插入检测,使用推杆电机控制测试卡的移动,配合硅光二极管实现对测试卡的扫描。结果实践表明,该仪器精密度上CV值不大于5%,低值与中值的准确性方面偏差不大于5%,在线性范围内梯度液相关系数大于0.99,光路零部件固定良好,运动机构简单,带有插卡自动检测、废卡抛样等功能。结论仪器采用的新设计是有效的,在食品安全、动物疫病等免疫荧光实验领域可广泛应用。Objective To design a miniaturized, low-cost immunofluorescence analyzer with automatic discarding function.Methods The immunofluorescence analyzer was designed with a non-confocal optical path. The light source was located on both sides of the optical module, and the photodiode was located directly above the test strip. The optical module used micro switch to detect the insertion of test trip, used push-rod motor to control the movement of the test strip, and realized the scanning process of the test card with the silicon photodiode. Results The results showed that the precision of the analyzer had a CV value of less than 5%, the accuracy of the low-value and intermediate-value was not more than 5%, and the gradient fluid correlation coefficient was more than 0.99 in the linear range. The immunofluorescence analyzer had well fixed optical module,simple movement mechanism, with the functions of automatic detection of card insert and automatic discarding of waste card.Conclusion The new design of the instrument is effective, and can be widely applied in the immunofluorescence of food safety and animal diseases.

关 键 词：免疫荧光 光学模块 体外诊断 即时检测 

分 类 号：TH773[机械工程—仪器科学与技术]