下调miR-221对胃癌细胞SGC-7901增殖的影响及其相关机制研究  

Effect of down-regulation of miR-221 on proliferation of gastric cancer cell line SGC-7901 and its related mechanisms

作　　者：常威[1] 刘海鸿 薛海贵 CHANG Wei;LIU Hai-hong;XUE Hai-gui(Tumor Radiotherapy Department,Zibo Central Hospital,255036,China)

机构地区：[1]淄博市中心医院肿瘤放疗科,255036

出　　处：《现代消化及介入诊疗》2019年第4期361-365,369,共6页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：山东省科技攻关计划(20170200194)

摘　　要：目的探讨下调miR-221后对胃癌细胞SGC-7901增殖的影响及其相关机制。方法收集2015年6月至2017年7月我院就诊的48例胃癌患者的癌旁组织、癌组织和32例同期体检健康者的正常胃组织,采用Real-time Q-PCR检测miR-221的表达水平。体外培养胃癌细胞SGC-7901,根据处理的方法不同分为组1(mimics对照组)、组2(hsa-miR-221组)、组3(antimiR组)、组4(anti-miR-221组),运用Real-time Q-PCR检测四组miR-221的表达,并通过噻唑蓝(MTT)比色法检测细胞的增殖,同时运用免疫印迹法(Western blotting)检测细胞增殖的相关信号通路STAT3蛋白的改变。结果胃癌组织miR-221阳性表达率显著高于癌旁组织、正常组织(均P <0. 05),癌旁组织miR-221阳性表达率高于正常组织,但差异无统计学意义(P> 0. 05)。胃癌SGC-7901细胞中的miR-221表达水平高于正常胃上皮细胞(4. 56±1. 21 vs. 15. 32±2. 74),差异有统计学意义(P <0. 01)。组2的增殖能力最强,组4的增殖能力最弱,组1和组3两组无明显差异(P> 0. 05)。四组细胞中STAT3蛋白的相对表达量分别为0. 341±0. 028、0. 748±0. 052、0. 376±0. 031、0. 218±0. 022,组2的STAT3蛋白表达水平最高,组4 STAT3表达水平最低,组1和组3两组无明显差异(P> 0. 05)。结论胃癌组织和胃癌细胞中miR-221的表达明显升高,下调miR-221可抑制胃癌细胞SGC-7901的增殖,其机制可能与下调STAT3蛋白有关。Objective To investigate the effect of down-regulation of miR-221 on the proliferation of gastric cancer cell line SGC-7901 and its related mechanisms.Methods The normal gastric tissues of 48 patients with gastric cancer who were treated in the oncology department of our hospital from June 2015 to July 2017 were collected,the miR-221 expression level was detected by Real-time Q-PCR.The gastric cancer cell SGC-7901 was cultured in vitro and according to different treatment methods,they were divided into group 1(mimics control group),group 2(hsa-miR-221 group),group 3(anti-miR group),group 4(anti-miR-221).The expression of miR-221 cells was inhibited by transient transfection of anti-miR-221(test group).The expression of miR-221 was detected by Real-time Q-PCR,and meanwhile the proliferation of the cells was detected by MTT colorimetric assay,and the changes of STAT3 protein related to cell proliferation were detected by Western blotting.Results The positive expression rate of miR-221 in gastric cancer tissues was significantly higher than that in adjacent normal tissues and normal tissues(P<0.05).The positive expression rate of mR-221 was higher than that of normal tissues,but the difference was not statistically significant(P>0.05).Differences in miR-221 expression between normal gastric epithelial cells and gastric cancer cells The expression level of miR-221 in gastric cancer SGC-7901 cells was higher than that in normal gastric epithelial cells[(4.56±1.21)ratio(15.32±2.74)],the difference was not statistically significant(P<0.01).Differences in cell proliferation and STAT3 protein expression between the four groups The relative expression levels of STAT3 protein in the four groups were(0.341±0.028),(0.748±0.052),(0.376±0.031)and(0.218±0.022),and the expression level of STAT3 protein was the highest in group 2,and the expression level of STAT3 in group 4 was the highest.At the lowest,there was no significant difference between group 1 and group 3(P>0.05).Conclusion The expression of miR-221 was significan

关 键 词：微小RNA 胃癌细胞 增殖 机制 

分 类 号：R735.2[医药卫生—肿瘤]