探讨As_2O_3诱导人胃癌SGC-7901细胞凋亡过程中AKT相关抗凋亡通路表达情况  

Explore the AKT Related Anti-apoptotic Pathways Expression in Arsenic Trioxide Induced Apoptosis of Human Gastric Cancer SGC-7901 Cells

作　　者：马瑞金 韩波[1] 杨宇[2] 林珊[1] 李瑛[1] MA Rui-jin;HAN Bo;YANG Yu;UN Shan;LI Ying(Department of Oncology The Grst AfGliated Hospital of Harbin Medical University,Harbin,Heilongjiang,150001,China;Department of Oncology The second ASiliated Hospital of Harbin Medical University,Harbin,Heilongjiang,150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院肿瘤二科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第二医院肿瘤科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2019年第5期816-819,871,共5页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金面上项目(H2016041)

摘　　要：目的:探讨三氧化二砷诱导胃癌细胞凋亡过程中,对AKT相关抗凋亡通路表达的影响。方法:分别用不同浓度(0μmol/L、7.5μmol/L、10μmol/L、12.5μmol/L和15μmol/L)三氧化二砷(As_2O_3)溶液处理胃癌细胞48 h,倒置显微镜观察细胞凋亡情况,并用Western blot方法检测p70S6kα、p-p70S6kα、p70S6kβ(S6蛋白激酶β,Ribosomal Protein S6 Kinaseβ)、p-p70S6kβ、rpS6、p-rpS6、p-BAD、BAD、p-GSK3β、GSK3β及NF-κB2等蛋白的表达情况与As_2O_3作用浓度之间的关系。结果:随As_2O_3作用细胞的浓度增大,p70S6kα、p-p70S6kα、p70s6kβ、p-p70S6kβ、p-BAD、p-GSK3β、NF-κB2蛋白的表达减少,rpS6、p-rpS6、GSK3β蛋白表达增多,各组间数据经统计学分析,P值均<0.05,具有统计学意义。BAD蛋白的表达无明显改变,P>0.05。结论:As_2O_3诱导人胃癌SGC-7901细胞凋亡机制中包括AKT相关的多个抗凋亡途径的激活,AKT在其中发挥重要作用。Objective:Explore the AKT related anti-apoptotic pathways expression in Arsenic trioxide induced apoptosis of human gastric cancer SGC-7901 cells.Methods:The gastric cancer cells were treated with different concentration(0μmol/L,7.5μmol/L,10μmol/L,12.5μmol/L and 15μmol/L)of As2O3 for 48 h.Cell apoptosis was observed by inverted microscope,the relationship between the expression of p70 S6 kα,p-p70 S6 kα,p70 S6 kβ,p-p70 S6 kβ,rpS6,p-rps6,p-BAD,BAD,p-GSK3β,GSK3β,NF-κB2 and the concentration of As2O3 was detected by Western blot.Results:Using Western blot assay to detect protein level,we founded that the expression of p70 S6 kα,p70 S6 Kβ,p-p70 S6 kα,p-p70 S6 kβ,p-BAD,p-GSK3β,NF-κB2 decreased,the expression of rpS6,p-rpS6,GSK3βexpression increased,Statistical analysis of each group of data showed that the P value was<0.05,with statistical significance.The expression of BAD protein had no obvious change with the concentration of As2O3 cells increased,the P value was>0.05,with no statistical significance.Conclusion:As2O3 induces the activation of multiple anti-apoptotic pathways related to AKT in the apoptosis mechanism of human gastric cancer SGC-7901,and AKT plays an important role in it.

关 键 词：三氧化二砷 胃肿瘤 细胞凋亡 蛋白激酶B 

分 类 号：R-33[医药卫生] R735.2