沉默miR-222上调PUMA基因表达诱导高转移潜能腺样囊性癌细胞凋亡  

Silencing miR-222 up-regulates PUMA expression and induces apoptosis in highly metastatic adenoid cystic carcinoma cells

作　　者：周子亮 曾秉辉[2] 魏常博 余东升[2] ZHOU Zi-liang;ZENG Bing-hui;WEI Chang-bo;YU Dong-sheng(Department of Stomatology, Xiangyang Hospital of Chancheng District, Foshan 528000, China)

机构地区：[1]佛山市禅城区向阳医院口腔科,主治医师广东528000 [2]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室,博士生广东510055

出　　处：《中华老年口腔医学杂志》2019年第2期65-69,共5页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金(项目编号:81472526);广东省科技计划项目(项目编号:2016A020216007);佛山市科技计划项目(项目编号:2017AB002271)

摘　　要：目的:研究沉默miR-222靶向p53上调凋亡调控因子(PUMA)对高转移潜能腺样囊性癌细胞系SACC-LM的影响。方法:用As-miR-222和对照(As-miR-222 NC)分别转染SACC-LM细胞,并设立空白对照,进行Transwell、CCK-8和流式细胞术,观察miR-222沉默对SACC-LM细胞迁移、增殖、凋亡和周期分布的影响。通过实时荧光定量PCR (qRT-PCR)与蛋白印迹测定(Western Blot)证实沉默miR-222靶向PUMA表达。结果:转染As-miR-222可抑制SACC-LM细胞的迁移和增殖,增加细胞凋亡率,并使G0/G1期的细胞比例显著增加(P<0. 01)。qRT-PCR与蛋白印迹实验证实,miR-222的沉默通过上调PUMA表达参与上述行为的调节。结论:沉默miR-222通过上调PUMA抑制SACC-LM细胞的迁移、增殖,诱导凋亡。Objective: To study the effect of silencing miR-222 targeting p53 up-regulation of apoptosis regulator (PUMA) on highy metastatic adenoid cystic carcinoma cell line SACC-LM. Methods: SACC-LM cells were transfected with As-miR- 222 and As-miR-222 NC respectively. The blank group was set up simultaneously. The migration was detected by Transwell assay. The proliferation was detected by CCK-8 assay. Apoptosis and cell cycle were examined by flow cytometry. The expressions of miR-222 and PUMA were analyzed by qRT-PCR and Western Blot. Results: Transfection of As-miR-222 inhibited the migration and proliferation of SACC-LM cells, increased the rate of apoptosis, and significantly increased the proportion of cells in G0/G1 phase (P<0. 01). MiR-222 was down-regulated significantly (P<0.05), while PUMA was up-regulated as confirmed by RT-PCR and Western Blot (P<0.01). Conclusions: Silencing miR-222 inhibits the migration and proliferation, indaces apoptosis of SACC-LM cells by up-regulating PUMA.

关 键 词：MIR-222 PUMA 高转移潜能 腺样囊性癌 凋亡 

分 类 号：R782[医药卫生—口腔医学]