血管内皮生长因子C和皮层肌动蛋白在食管鳞癌细胞中的表达及其临床意义  

Expressions of vascular endothelial growth factor C and cortactin in esophageal squamous cell carcinoma and their clinical significance

作　　者：杨阳[1] 李校天[2] 张九娜[2] 周丽云[2] 郭永泽[2] YANG Yang;LI Xiao-tian;ZHANG Jiu-na;ZHOU Li-yun;GUO Yong-ze(Clinical College, Hebei University of Engineering,Handan 056002,Hebei,China;Department ofGastroenterology,the Affiliated Hospital of Hebei University of Engineering,Handan 056002,Hebei,China)

机构地区：[1]河北工程大学临床学院,邯郸医学硕士056002 [2]河北工程大学附属医院消化内科,邯郸056002

出　　处：《医学研究生学报》2019年第5期501-505,共5页Journal of Medical Postgraduates

基　　金：河北省科技计划项目(16277742D)

摘　　要：目的血管内皮生长因子C(VEGFC)、皮层肌动蛋白(CTTN)与食管鳞癌的生长密切相关,但其具体作用关系尚未明确。文中旨在通过体外分别干扰人食管鳞癌TE1细胞中VEGFC、CTTN的基因表达,明确两因子对食管鳞癌细胞增殖及凋亡的影响。方法实验根据添加不同试剂分为6组:空白组(单纯培养细胞)、MATE转染组(添加MATE试剂)、阴性对照组(添加阴性对照RNA及MATE试剂)、阳性对照组(添加阳性对照RNA及MATE试剂)、VEGFCsiRNA转染组(添加VEGFC siRNA及MATE试剂)、CTTNsiRNA转染组(添加CTTNsiRNA及MATE试剂)。采用CCK8检测各组细胞增殖情况,流式细胞仪检测细胞凋亡情况。结果 VEGFCsiRNA转染组VEGFC mRNA表达量(0.13±0.01)、CTTNsiRNA转染组CTTN mRNA表达量(0.29±0.02)较空白组(1.00±0.00、1.00±0.00)明显降低(P<0.05)。VEGFCsiRNA转染组、CTTNsiRNA转染组细胞增殖率较其他组明显降低(P<0.05),VEGFCsiRNA转染组细胞增殖率[(31.26±5.25)%]较CTTNsiRNA转染组[(46.99±4.82)%]明显降低(P<0.05)。CTTNsiRNA转染组细胞凋亡率[(36.78±4.29)%]较VEGFCsiRNA转染组[(48.41±5.37)%]明显降低(P<0.05)。结论干扰VEGFC、CTTN表达均可抑制食管鳞癌细胞增殖并促进其凋亡,从而抑制食管鳞癌细胞生长;VEGFC较CTTN对食管鳞癌细胞的增殖及凋亡影响作用更为显著。Objective Vascular endothelial growth factor C(VEGFC)and cortactin(CTTN)have been found to be closely related to the growth of esophageal squamous cell carcinoma(ESCC),but their specific relationship has not been clearly defined up to the present time. This study aimed to investigate the effects of VEGFC and CTTN on the proliferation and apoptosis of ESCC cells. Methods Human ESCC TE1 cells were treated with normal culture medium(the blank control group),MATE transfection reagent ( the MATE group),negative control RNA and MATE reagent(the negative control group),positive control RNA and MATE reagent (the positive control group),VEGFC siRNA and MATE transfection reagent(the VEGFC siRNA group),and CTTN siRNA and MATE transfection reagent(the CTTN siRNA group). The proliferation of the ESCC TE1 cells in different groups was detected by CCK-8 assay and their apoptosis determined by flow cytometry. Results Compared with the blank control group,the ESCC cells of the VEGFC siRNA and CTTN siRNA groups showed significantly decreased expressions of VEGFC mRNA(1.00 ± 0.00 vs 0.13 ± 0.01,P < 0.05)and CTTN mRNA (1.00 ± 0.00 vs 0.29 ± 0.02,P < 0.05). The proliferation rate of the ESCC cells was remarkably lower in the VEGFC siRNA and CTTN siRNA than in the other groups(P < 0.05), and even lower in the VEGFC siRNA than in the CTTN siRNA group([31.26 ± 5.25]% vs[46.99 ± 4.82]%,P < 0.05),but their apoptosis rate was markedly higher in the former than in the latter group([48.41 ± 5.37]% vs[36.78 ± 4.29]%,P < 0.05). Conclu? sion Interfering with the expressions of VEGFC and CTTN can inhibit the proliferation and promote the apoptosis of ESCC cells,and VEGFC has an even better effect than CTTN.

关 键 词：食管鳞癌 血管内皮生长因子C 皮层肌动蛋白 

分 类 号：R735.1[医药卫生—肿瘤]