神经免疫指标与孤独症谱系障碍的关联  

Association between neuroimmunological indicators and autism spectrum disorders

作　　者：王琳 杨晓蕾[2] 马永娟 刘晓翠 邹明扬 孙彩虹 武丽杰 WANG Lin;YANG Xiaolei;MAYongjuan;LIU Xiaocui;ZOU Mingyang;SUN Caihong;WU Lijie(Department of Child and Adolescent Health, Public Health College,Harbin Medical University, Harbin(150081), China)

机构地区：[1]哈尔滨医科大学公共卫生学院儿少卫生与妇幼保健学教研室,黑龙江150081 [2]齐齐哈尔医学院公共卫生学院

出　　处：《中国学校卫生》2019年第4期499-502,共4页Chinese Journal of School Health

基　　金：国家重点基础研究发展计划项目(2012CB517901)

摘　　要：目的探讨骨桥蛋白(osteopontin, OPN)、白介素17A(interleukin-17A, IL-17A)、抗髓鞘碱性蛋白抗体(anti-MBP auto-antibody)与孤独症谱系障碍(autism spectrum disorder, ASD)的关系,为ASD的病因及发病机制研究提供理论依据。方法采用病例对照研究方法,收集哈尔滨市孤独症康复定点机构进行康复训练的ASD儿童40例作为病例组,1∶1性别、年龄匹配的正常儿童作为对照组。应用酶联免疫吸附试验(ELISA)方法检测两组儿童血清OPN,IL-17A,anti-MBP auto-antibody水平,并采用Pearson或Spearman相关与ASD儿童严重程度及智力水平进行关联分析。结果 ASD组血清OPN,IL-17A水平[(296.89±162.95),0.93]pg/mL均高于对照组[(217.98±113.39),0.62]pg/mL(P值均<0.05)。ASD组血清OPN,IL-17A,anti-MBP auto-antibody水平与儿童孤独症行为量表(ABC),儿童孤独症评定量表(CARS),皮博迪图片词汇测验(PPVT)得分相关均无统计学意义(P值均>0.05),ASD组血清anti-MBP auto-antibody与OPN,IL-17A均呈正相关(r值分别为0.35,0.34,P值均<0.05)。结论 ASD儿童存在明显的神经免疫异常,机制有待进一步探讨。Objective To explore the relationship among osteopontin(OPN), Interleukin-17 A(IL-17 A), anti-MBP auto-antibody and autism spectrum disorder(ASD), and to provide the theoretical basis for the etiology and pathogenesis of ASD. Methods Forty autistic children and forty matched healthy children were enrolled in this case-control study. The levels of OPN, IL-17 A, anti-MBP auto-antibody in serum were measured by enzyme-linked immunosorbent assay(ELISA). The associations between those metabolic levels and the severity and intelligence of ASD children were performed by Pearson or Spearman correlation. Results Children with ASD had higher serum levels of OPN, IL-17 A [(296.89±162.95),0.93] pg/mL compared to healthy control[(217.98±113.39), 0.62] pg/mL(P<0.05). Serum OPN, IL-17 A, and anti-MBP auto-antibody levels in ASD group were not correlated with the scores of ABC, CARS, and PPVT(P>0.05). However, anti-MBP auto-antibodies level in children with ASD were positively correlated with OPN and IL-17 A levels, respectively(r=0.35, 0.34, P<0.05). Conclusion It was obvious that the ASD children were found with neuroimmunologic abnormality, and the underlying mechanism needs to be further explored.

关 键 词：骨桥蛋白质 白细胞介素17 孤独性障碍 病例对照研究 儿童 

分 类 号：R749.94[医药卫生—神经病学与精神病学] R446.1[医药卫生—临床医学]